LOCUS       sequence-114019-        1491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AREL1 HECT domain in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINS-AREL1
FEATURES             Location/Qualifiers
     source          1..1491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtagca gccatcacca tcatcatcac gggagcgata gcgaagtgaa ccaggaagcg
       61 aaaccggaag ttaaaccgga agtgaaaccg gaaacccata ttaatctgaa agttagcgac
      121 ggcagcagcg aaatcttttt taaaattaaa aaaaccaccc cgctgcgtcg cctgatggaa
      181 gcctttgcga aacgtcaggg taaagaaatg gatagcctgc gctttctgta tgacggcatc
      241 cgtattcagg ccgatcagac cccggaagac ctggatatgg aagacaacga tattattgaa
      301 gcgcatcgcg aacagatcgg tggttctgag acctttcagg acaaggtgaa ctttttccag
      361 cgagagcttc ggcaggtaca tatgaaaaga ccacattcca aagtcaccct gaaggtcagc
      421 agacatgcct tgttggaatc gtctctgaaa gccactcgga atttctccat ctcagattgg
      481 agcaagaact ttgaggttgt tttccaggat gaagaagctc tggactgggg agggcctcgc
      541 cgggaatggt ttgagctaat ctgcaaagca ctatttgata caaccaatca gctcttcacc
      601 cggttcagtg acaacaacca agcattagtg catcccaacc ctaatcgccc cgctcatctg
      661 cgcctgaaaa tgtatgagtt tgcgggacgg ctcgtgggca agtgtctcta tgagtcctct
      721 ctaggaggag cctacaagca gttggtccga gctcgcttca cccgctcttt cctggcccaa
      781 atcataggac tgcgtatgca ttacaagtac tttgaaacag atgacccaga attctacaaa
      841 tctaaagttt gttttatcct caacaatgac atgagtgaga tggagctggt ctttgcagaa
      901 gagaaatata ataaatcagg tcaattggat aaggttgtag aactcatgac aggtggagct
      961 caaactccag tcaccaatgc gaataaaatc ttctatttaa atttgctggc ccaatatcgg
     1021 ctggccagtc aagtgaaaga ggaggtggaa catttcctaa aaggcctgaa tgaattggtc
     1081 cctgagaacc ttttggctat ttttgatgag aatgagcttg agctgctgat gtgtgggact
     1141 ggagacatca gtgtgtctga cttcaaagcc catgcagtag ttgttggtgg ctcatggcat
     1201 ttcagagaaa aggtcatgag gtggttttgg actgtggttt ccagtctgac ccaggaggag
     1261 ttggctcggc tacttcagtt cacaacaggc tcctctcagc taccacctgg aggctttgcc
     1321 gccctctgtc cctcatttca gattattgcc gctccgaccc atagcacgct gcctactgca
     1381 cacacatgtt ttaaccagct gtgcctccct acatatgact cctatgaaga ggtgcacagg
     1441 atgctgcagc tggccatcag cgagggttgc gagggctttg gcatgctctg a
//
