LOCUS       sequence-114021-        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses activated AMSH in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINB-AMSH*
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..63
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggtc tggaagttct gtttcagggc
       61 ccgactgcca acccgttcga gcaagatgtg gaaaaagcca caaatgagta caacaccaca
      121 gaagactgga gtctgatcat ggacatctgt gacagggttg gaagcactcc cagtggagca
      181 aaagattgcc taaaagccat aatgaaaagg gtaaaccata aggtccctca cgttgctctg
      241 caggcattga cccttcttgg agcttgtgtg gcaaactgtg gaaagatatt tcatttagaa
      301 gtatgttccc gtgattttgc aacggaagta cgttctgtga taaaaaataa ggctcatcct
      361 aaagtatgtg aaaaactaaa atctctaatg gtggaatggt cagaagaatt ccagaaggac
      421 ccccaattta gtctgatatc tgcaactatt aagtctatga aagaagaagg ggttactttt
      481 ccttccgcag gctcccagac tgtcgcggct gctgccaaaa acgggacatc attgaacaaa
      541 aacaaagaag atgaggacat agctaaagct attgagttat cgttgcaaga gcagaagcag
      601 cagtacacgg agacaggcgg cagcagcggc ggcagcaact cagaaagtat tcccacaatc
      661 gatggattgc gccatgtggt ggtgcctggg cggctgtgcc cacagtttct ccagttagcc
      721 agtgccaaca ctgcccgggg agtggagaca tgtggaattc tctgtggaaa actgatgagg
      781 aatgaattta ccattaccca tgttctcatc cccaagcaaa gtgctgggtc tgattactgc
      841 aacacagaga acgaagaaga acttttcctc atacaggatc agcagggcct catcacactg
      901 ggctggattc atactcaccc cacacagacc gcgtttctct ccagtgtcga cctacacact
      961 cactgctctt accagatgat gttgccagag tcagtagcca ttgtttgctc ccccaagttc
     1021 caggaaactg gattctttaa actaactgac catggactag aggagatttc ttcctgtcgc
     1081 cagaaaggat ttcatccaca cagcaaggat ccacctctgt tctgtagctg cagccacgtg
     1141 actgttgtgg acagagcagt gaccatcaca gaccttcgat aa
//
