LOCUS       sequence-114036-        1926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NleL (170-782) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Hospenthal MK, Freund SM, Komander D
  TITLE     Assembly, analysis and architecture of atypical ubiquitin chains.
  JOURNAL   Nat Struct Mol Biol. 2013 May;20(5):555-65. doi: 10.1038/nsmb.2547. 
            Epub 2013 Apr 7.
  PUBMED    23563141
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2547";
            journalName: "Nat Struct Mol Biol"; date: "2013-05"; volume: "20"; 
            issue: "5"; pages: "555-65"
COMMENT     Sequence Label: pMCSG17-NleL
FEATURES             Location/Qualifiers
     source          1..1926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..57
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             58..84
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggcacacc atcaccacca tcacagcagc ggtctggaag ttctgtttca gggcccgtac
       61 ccatacgatg ttccagatta cgctagtcaa ggccgtgcct gcctgagcaa agctgagctt
      121 actgctgact taatttggtt gtcagctaac cgaacgggtg aagagtctgc tgaagaattg
      181 aattactctg gatgtgactt gtcaggtcta agtcttgtag ggctgaattt atcatcagta
      241 aatttttctg gagcagtgct tgatgataca gatctcagga tgagtgattt gtctcaggct
      301 gtattggaaa actgttcttt taaaaactcg attttgaatg aatgtaattt ttgttatgct
      361 aatttatcta attgtattat tagggctttg tttgaaaact ctaatttcag caattccaat
      421 cttaaaaatg catcatttaa aggatcttca tatatacaat atcctccaat tttgaacgag
      481 gctgatttaa caggagctat tataattcct ggaatggttt taagtggtgc tatcttaggt
      541 gatgtaaagg agctctttag tgaaaaaagt aataccatta atctaggagg gtgttacata
      601 gatctatctg acatacagga aaatatatta tctgtgttgg ataactatac aaaatcaaat
      661 aaatcaattt tattgactat gaatacatct gatgataagt ataaccatga taaagtaagg
      721 gccgctgaag aacttatcaa aaaaatatct cttgacgaat tagcggcgtt ccggccctat
      781 gttaagatgt ctttggctga ttcatttagt attcatcctt atttgaacaa cgcaaatata
      841 cagcaatggc tcgagcctat atgtgatgac ttttttgata ctataatgtc ttggtttaat
      901 aattcaataa tgatgtatat ggagaatggt agtttattgc aggcagggat gtattttgag
      961 cgacatccag gtgcgatggt atcttataat agttccttta tacaaattgn aatgaatggt
     1021 tcacggcgtg atggaatgca ggaacgattt agggaactct atgaagtata tttaaaaaat
     1081 gaaaaagttt atcctgtcac acagcagagt gattttggat tgtgcgatgg ctctgggaag
     1141 cctgactggg atgatgattc cgatttggct tataactggg ttttgttatc atcacaggat
     1201 gatggtatgg caatgatgtg ttctttgagt catatggttg atatgttatc tcctaataca
     1261 tcaactaatt ggatgtcctt ttttttatat aaggatggag aagttcaaaa tacatttggg
     1321 tattcattga gcaatctttt ttctgaatca tttccaattt tcagtattcc ttatcataaa
     1381 gctttttccc agaatttcgt ttctggtatt ctggatatac tcatttctga taatgaactc
     1441 aaagagagat ttattgaggc acttaattcc aataaatcag attataaaat gattgctgat
     1501 gatcagcaaa ggaaacttgc ctgtgtctgg aatccctttc ttgatggttg ggaactgaac
     1561 gctcagcatg tagatatgat tatggggagc catgtattga aagatatgcc actaagaaaa
     1621 caggctgaaa tattattttg tttagggggg gttttctgta aatactcatc gagtgatatg
     1681 tttggtacag agtatgattc tcctgagatt ctacggagat atgcaaatgg attgattgaa
     1741 caagcttata aaacagatcc tcaggtattt ggctcagttt attattacaa tgatatttta
     1801 gacaggctac aaggaagaaa taatgttttt acttgtaccg ctgtgctgac tgatatgcta
     1861 acggagcatg caaaagaatc ttttcctgaa atattttcat tgtattatcc tgttgcgtgg
     1921 cgttga
//
