LOCUS       sequence-114038-         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli Genetic Modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pREDKI
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
       61 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
      121 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
      181 acctgtcgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
      241 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
      301 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
      361 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
      421 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
      481 tgccgcgcct gcgccatccc catgctacct aagcgccagt gtggttgccc tgcgctggcg
      541 ttaaatcccg gaatcgcccc ctgccantca agattcagct tcagacgctc cgggcaataa
      601 ataatattct gcaaaacc
//
