LOCUS       sequence-114039-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + ColE1 + PLtetO-1:: extra 5'UTR::RBS(st7):cfp::T1 terminator +
            PLlacO-1::RBS(st7):psu::T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Hussein R, Lee TY, Lim HN
  TITLE     Quantitative characterization of gene regulation by Rho dependent 
            transcription termination.
  JOURNAL   Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 
            10.1016/j.bbagrm.2015.05.003.
  PUBMED    25982507
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2015.05"; journalName: "Biochim Biophys Acta"; 
            date: "2015-05-14- 14"; volume: "1849"; issue: "8"; pages: "940-954"
COMMENT     Sequence Label: pHL 2067
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      45..131
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    182..198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      827..873
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agtaaggatc ctatgaagct tcatggtacg cgtgctagag gcatcaaata aaacgaaagg
       61 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      121 gtaggacaaa tccgccgccc tagagaattc gatccataaa tgtgagcgga taacattgac
      181 attgtgagcg gataacaaga tactcccggg taaggaggaa aaaaaatgga aagcacagcc
      241 ttacagcagg cctttgacac ctgtcagaat aacaaagcag catggctgca acgcaaaaat
      301 gagctggcag cggccgaaca ggaatatctg cggcttctgt caggagaagg cagaaacgtc
      361 agtcgcctgg acgaattacg caatattatc gaagtcagaa aatggcaggt gaatcaggcc
      421 gccggtcgtt atattcgttc gcatgaagcc gttcagcaca tcagcatccg cgaccggctg
      481 aatgatttta tgcagcagca cggcacagca ctggcggccg cactggcacc ggagctgatg
      541 ggctacagtg agctgacggc cattgcccga aactgtgcca tacagcgtgc cacagatgcc
      601 ctgcgtgaag cccttctgtc ctggcttgcg aagggtgaaa aaattaatta ttccgcacag
      661 gatagcgaca ttttaacgac catcggattc aggcctgacg tggcttcggt ggatgacagc
      721 cgtgaaaaat tcacccctgc gcagaacatg attttttcgc gtaaaagtgc gcaactggca
      781 tcacgtcagt cagtgtaagg gcccatggta cgcgtgctag aggcatcaaa taaaacgaaa
      841 ggctcagtcg aaagactggg cctttcgttt tatct
//
