LOCUS       sequence-11404-e       11314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T
  TITLE     GSK3beta phosphorylation modulates CLASP-microtubule association and
            lamella microtubule attachment.
  JOURNAL   J Cell Biol. 2009 Mar 23. 184(6):895-908.
  PUBMED    19289791
REFERENCE   2  (bases 1 to 11314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Mar 23. 184(6):895-908."
COMMENT     Sequence Label: pShuttle-EGFP-CLASP2 340-1362 9xS/A
FEATURES             Location/Qualifiers
     source          1..11314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        399..702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        703..906
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     857..877
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      946..955
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(952..954,955..957,958..1668)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(952..954,955..957,958..1668)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(997..1018)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1258..1277)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1605..1626
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             4377..4385
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4962..5083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4999..5018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5053..5072
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8274..8376
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(8465..8482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8636..9224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8716..8735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10056..10850
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10056..10850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10110..10129)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10720..10739
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(10898..11277)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10960..10981)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11172..11191
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      901 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgccac catggtgagc
      961 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1021 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1081 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1141 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1201 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1261 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1321 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1381 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1441 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1501 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1561 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1621 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ctcagatctc
     1681 gagctcaagc ttcgaattca tgatgctgac gctgaggcca gagtggaggc aagaaagaca
     1741 tacatgggtc ttagaaacca ctttcctggt gaagctgaaa cattatataa ttcccttgag
     1801 ccatcttatc agaagagtct tcaaacttac ttaaagagtt ctggcagtgt agcatctctt
     1861 ccacaatcag acaggtcctc atccagctca caggaaagtc tcaatcgccc tttttcttcc
     1921 aaatggtcta cagcaaatcc atcaactgtg gctggaagag tatcagcagg cagcagcaaa
     1981 gccagttccc ttccaggaag cctgcagcgt tcacgaagtg acattgatgt gaatgctgct
     2041 gcaggtgcca aggcacatca tgctgctgga cagtctgtgc gaagggggcg cttaggtgca
     2101 ggtgccctga atgcaggttc ctatgcgtca ctagaggata cttctgacaa gctggatgga
     2161 acagcatctg aagatggccg ggtgagagca aaactttcag caccacttgc tggcatggga
     2221 aatgccaagg cagattctag aggaagaagt cgaacaaaaa tggtgtctca atcacagcct
     2281 ggtagccggt ctgggtctcc aggaagagtt ctgaccacaa cagccctgtc cactgtgagc
     2341 tctggtgttc aaagagtcct ggtcaattca gcctcagcac aaaaaagaag caagatacca
     2401 cgggcccagg gctgtgccag agaggctgct ccatctaggc tttcagtggc ccgaagcagt
     2461 cgtattcctc gaccaagtgt ggctcaagga tgcgcccggg aagctgctcg ggagagcgcc
     2521 agagacacag ctcctgttcg ctcttttcag cccctcgcct ccagacacca ttccagatca
     2581 actggtgccc tctacgcccc cgaagtgtat ggggcctcag gtccaggtta tgggatcagc
     2641 caatcaagtc gactgtcgtc ttctgttagt gccatgcgag tcctgaacac aggttctgat
     2701 gtggaggagg cggtggcaga tgccttgaaa aaaccagctc gaagaagata tgaatcatat
     2761 ggaatgcatt cagatgatga cgccaacagc gatgcatcta gtgcttgttc agaacgctcc
     2821 tatagttctc gaaatggtag tattcctaca tatatgaggc agacggaaga tgtggcagaa
     2881 gtcctcaata gatgtgctag ttccaattgg tcagaaagga aagaaggcct cctaggtctg
     2941 cagaacttat taaaaaatca gagaacacta agtcgagttg aactgaaaag attatgtgaa
     3001 attttcacaa gaatgtttgc tgaccctcat ggcaagagag tattcagcat gtttttggag
     3061 actctagtgg atttcataca agtccacaaa gatgatcttc aagattggtt gtttgtactg
     3121 ctgacacaac tactaaaaaa aatgggtgct gatttgcttg gatctgttca ggcaaaagtt
     3181 cagaaagccc ttgatgttac aagagagtct tttccaaatg atcttcagtt caatattcta
     3241 atgagattta cagttgatca gacccagaca ccaagcttaa aggtgaaggt tgctatcctt
     3301 aaatacatag aaactctggc caaacagatg gatccaggag attttataaa ttccagtgaa
     3361 actcgcctag cagtgtctcg ggtcatcact tggacaacag aacccaaaag ttctgatgtt
     3421 cggaaggcag cacagtcagt gctgatttca ttatttgaac tcaatacccc agagtttaca
     3481 atgttattag gagctttacc aaaaactttt caggatggtg ctaccaagct tcttcataat
     3541 caccttcgaa acactggcaa tggaacccag agttccatgg ggagtccttt gacaagacca
     3601 acaccacgat caccagctaa ctggtccagt cctcttactt ctcctaccaa tacatcacag
     3661 aatactttat ctccaagtgc atttgattat gacacagaaa atatgaactc tgaagatatt
     3721 tatagctctc ttagaggtgt cactgaagca atccagaatt tcagcttccg tagccaagaa
     3781 gatatgaatg agccattgaa aagggattct aaaaaagatg atggcgattc aatgtgtggt
     3841 ggtcctggga tgtctgaccc aagagcagga ggtgatgcta ctgactcaag tcaaacagct
     3901 cttgataata aagcttcatt gctccattca atgcctactc actcctctcc acgctctcga
     3961 gactataatc catataacta ttcagatagc atcagtccct tcaacaagtc tgccctcaag
     4021 gaagccatgt ttgatgatga tgctgaccag tttcctgacg atctttccct agatcattct
     4081 gacctagttg cagagttgtt gaaggagctg tctaaccata atgagcgtgt agaagaaaga
     4141 aaaattgccc tctatgaact tatgaaactg acacaggaag aatcttttag tgtttgggat
     4201 gaacacttca aaacaatatt gcttttattg cttgaaacgc ttggagataa agagcctaca
     4261 atcagggctt tggcattaaa ggttttaaga gaaatcctaa ggcatcaacc agcaagattt
     4321 aaaaactatg cagaattgac tgtcatgaaa acattggaag cacataaaga tcctcataag
     4381 gaggtggtga gatctgctga ggaagcggca tcagtgttgg ccacttcaat tagtccagag
     4441 cagtgcatca aagtgctttg tcctatcatt caaactgcag actacccaat taatctggct
     4501 gcaatcaaaa tgcaaacaaa agtgatagag agagtgtcca aggaaaccct aaacctgctt
     4561 ttgccagaga ttatgccagg tctaatacag ggttatgata attcagagag cagtgttcgg
     4621 aaagcttgtg tcttctgcct ggtggctgtt catgcggtaa ttggtgatga actaaaacca
     4681 catctcagtc aacttactgg cagtaaaatg aagctactga atctttacat caaacgtgca
     4741 caaacaggtt ctggaggagc tgatcccact actgatgttt ctggacaaag tgataggcgg
     4801 ccgctcgagc ctaagcttct agataagata tccgatccac cggatctaga taactgatca
     4861 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4921 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4981 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5041 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgga tctgggcgtg
     5101 gttaagggtg ggaaagaata tataaggtgg gggtcttatg tagttttgta tctgttttgc
     5161 agcagccgcc gccgccatga gcaccaactc gtttgatgga agcattgtga gctcatattt
     5221 gacaacgcgc atgcccccat gggccggggt gcgtcagaat gtgatgggct ccagcattga
     5281 tggtcgcccc gtcctgcccg caaactctac taccttgacc tacgagaccg tgtctggaac
     5341 gccgttggag actgcagcct ccgccgccgc ttcagccgct gcagccaccg cccgcgggat
     5401 tgtgactgac tttgctttcc tgagcccgct tgcaagcagt gcagcttccc gttcatccgc
     5461 ccgcgatgac aagttgacgg ctcttttggc acaattggat tctttgaccc gggaacttaa
     5521 tgtcgtttct cagcagctgt tggatctgcg ccagcaggtt tctgccctga aggcttcctc
     5581 ccctcccaat gcggtttaaa acataaataa aaaaccagac tctgtttgga tttggatcaa
     5641 gcaagtgtct tgctgtcttt atttaggggt tttgcgcgcg cggtaggccc gggaccagcg
     5701 gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg tggtaaaggt gactctggat
     5761 gttcagatac atgggcataa gcccgtctct ggggtggagg tagcaccact gcagagcttc
     5821 atgctgcggg gtggtgttgt agatgatcca gtcgtagcag gagcgctggg cgtggtgcct
     5881 aaaaatgtct ttcagtagca agctgattgc caggggcagg cccttggtgt aagtgtttac
     5941 aaagcggtta agctgggatg ggtgcatacg tggggatatg agatgcatct tggactgtat
     6001 ttttaggttg gctatgttcc cagccatatc cctccgggga ttcatgttgt gcagaaccac
     6061 cagcacagtg tatccggtgc acttgggaaa tttgtcatgt agcttagaag gaaatgcgtg
     6121 gaagaacttg gagacgccct tgtgacctcc aagattttcc atgcattcgt ccataatgat
     6181 ggcaatgggc ccacgggcgg cggcctgggc gaagatattt ctgggatcac taacgtcata
     6241 gttgtgttcc aggatgagat cgtcataggc catttttaca aagcgcgggc ggagggtgcc
     6301 agactgcggt ataatggttc catccggccc aggggcgtag ttaccctcac agatttgcat
     6361 ttcccacgct ttgagttcag atggggggat catgtctacc tgcggggcga tgaagaaaac
     6421 ggtttccggg gtaggggaga tcagctggga agaaagcagg ttcctgagca gctgcgactt
     6481 accgcagccg gtgggcccgt aaatcacacc tattaccggc tgcaactggt agttaagaga
     6541 gctgcagctg ccgtcatccc tgagcagggg ggccacttcg ttaagcatgt ccctgactcg
     6601 catgttttcc ctgaccaaat ccgccagaag gcgctcgccg cccagcgata gcagttcttg
     6661 caaggaagca aagtttttca acggtttgag accgtccgcc gtaggcatgc ttttgagcgt
     6721 ttgaccaagc agttccaggc ggtcccacag ctcggtcacc tgctctacgg catctcgatc
     6781 cagcatatct cctcgtttcg cgggttgggg cggctttcgc tgtacggcag tagtcggtgc
     6841 tcgtccagac gggccagggt catgtctttc cacgggcgca gggtcctcgt cagcgtagtc
     6901 tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg ccagggtgcg cttgaggctg
     6961 gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg cgtcggccag gtagcatttg
     7021 accatggtgt catagtccag cccctccgcg gcgtggccct tggcgcgcag cttgcccttg
     7081 gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg cgtagagctt gggcgcgaga
     7141 aataccgatt ccggggagta ggcatccgcg ccgcaggccc cgcagacggt ctcgcattcc
     7201 acgagccagg tgagctctgg ccgttcgggg tcaaaaacca ggtttccccc atgctttttg
     7261 atgcgtttct tacctctggt ttccatgagc cggtgtccac gctcggtgac gaaaaggctg
     7321 tccgtgtccc cgtatacaga cttgagaggg agtttaaacg aattcaatag cttgttgcat
     7381 gggcggcgat ataaaatgca aggtgctgct caaaaaatca ggcaaagcct cgcgcaaaaa
     7441 agaaagcaca tcgtagtcat gctcatgcag ataaaggcag gtaagctccg gaaccaccac
     7501 agaaaaagac accatttttc tctcaaacat gtctgcgggt ttctgcataa acacaaaata
     7561 aaataacaaa aaaacattta aacattagaa gcctgtctta caacaggaaa aacaaccctt
     7621 ataagcataa gacggactac ggccatgccg gcgtgaccgt aaaaaaactg gtcaccgtga
     7681 ttaaaaagca ccaccgacag ctcctcggtc atgtccggag tcataatgta agactcggta
     7741 aacacatcag gttgattcac atcggtcagt gctaaaaagc gaccgaaata gcccggggga
     7801 atacataccc gcaggcgtag agacaacatt acagccccca taggaggtat aacaaaatta
     7861 ataggagaga aaaacacata aacacctgaa aaaccctcct gcctaggcaa aatagcaccc
     7921 tcccgctcca gaacaacata cagcgcttcc acagcggcag ccataacagt cagccttacc
     7981 agtaaaaaag aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca
     8041 cagtgtaaaa aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg
     8101 gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag
     8161 ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtcacttcc
     8221 cattttaaga aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc
     8281 acccgccccg ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt
     8341 ggcttcaatc caaaataagg tatattattg atgatgttaa ttaacatgca tggatccata
     8401 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     8461 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8521 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8581 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8641 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8821 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9061 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9301 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9361 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9421 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9481 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9541 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9601 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9661 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcagccatga
     9721 gattatcaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac
     9781 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
     9841 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
     9901 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
     9961 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
    10021 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
    10081 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
    10141 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
    10201 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
    10261 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
    10321 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
    10381 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
    10441 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
    10501 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
    10561 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
    10621 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
    10681 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
    10741 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
    10801 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa
    10861 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcac catcccttat
    10921 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    10981 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    11041 ccactacgtg aaccatcacc ctaatcaagt tttttgtggt cgaggtgccg taaagatcta
    11101 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    11161 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    11221 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca
    11281 ttcgccattc aggatcgaat taattcttaa ttaa
//
