LOCUS       sequence-114040-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR + ColE1 + 
            PLlacO-1::RBS(st7):rfp::RBS(st7):cfp::RBS(st7):yfp::T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Hussein R, Lee TY, Lim HN
  TITLE     Quantitative characterization of gene regulation by Rho dependent 
            transcription termination.
  JOURNAL   Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 
            10.1016/j.bbagrm.2015.05.003.
  PUBMED    25982507
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2015.05"; journalName: "Biochim Biophys Acta"; 
            date: "2015-05-14- 14"; volume: "1849"; issue: "8"; pages: "940-954"
COMMENT     Sequence Label: pHL 309
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     127..150
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             join(261..263,264..266,267..779)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(306..327)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(567..586)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgccg
       61 aggtcaagac cacctacaag gccaagaagc ccgtgcagct tcccggcgcc tacaacgtca
      121 acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
      181 gcgccgaggg ccgccactca accggcggca tggacgagct gtacaagtaa ggatccatac
      241 ggccctaagg aggaaaaaaa atggtgagca agggcgagga gctgttcacc ggggtggtgc
      301 ccatcctggt cgagctggac ggcgacgtaa acggccacag gttcagcgtg tccggcgagg
      361 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      421 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      481 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      541 tccaggagcg taccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      601 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      661 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      721 ccgccgacaa gcagaagaac ggcatcaagg cccacttcaa gatccgccac aacatcgagg
      781 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      841 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      901 agaagcgcga tcacatg
//
