LOCUS       sequence-114042-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 1346.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H1346
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     23..85
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             533..559
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             611..628
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      660..707
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttacttttag gagataaaac atatgaaaaa gaccgcaatc gccatcgcag tggcactggc
       61 tggtttcgct accgtagcgc aggccgcgcc gaacgacaac acctggtata ctggtgctaa
      121 actgggctgg tcccagtacc acgacactgg tttcatcaat aataatggcc cgacccacga
      181 gaaccagcag ggtatcggtg cctttgcagg catccaggtg aacccgtacg ttggctttga
      241 aatgggttac gactggctcg gtcgtatgcc gtacaaaggc agcgttgaaa acggtgcata
      301 caaggctcag ggcgttcaac tgaccgctaa actgggttac ccgatcaccg acgacgttga
      361 cgtgtacctg cgtctgggca tcatggtatg gcgtgcagat actaaatcca acgtttatgg
      421 taaaaaccac gataccggcg tttctccggt cttcgctggc ggtgttgagt acgcgatcac
      481 tccggagatc gctatgcgtc tggaaacgca acacaccaac aacatcggtg actacccata
      541 cgatgttcca gattacgcga ctcgtccgga taactggatg gtgtctctgg gtgcgtccta
      601 ccgtttcggt tgttgccctg gttgctgtca ccatcaccac catcactaaa agcttccccc
      661 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgccc ctgagacgcg
      721 tcaatcgagt tcgtacctaa gggcgacacc ccataattag cccgggcgaa aggcccagtc
      781 tttcgactga gcctttcgtt tttatttgat gcctggcagt tccctactct cgca
//
