LOCUS       sequence-114043-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conservative version of OmpA Redesign 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_R4cons
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     18..80
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             528..554
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             606..623
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             624..641
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      655..702
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttaggagat aaaacatatg aaaaagaccg caatcgccat cgcagtggca ctggctggtt
       61 tcgctaccgt agcgcaggcc gcgccgaaag acaacacctt ctatctcggc ctgaaaatgg
      121 ggatcagcca gtatcacgat acgggattta ttaacaataa tggtccgacg catgaaaatc
      181 agctggggat cggcgcattt ggaggtatcc aggtcaatcc ctacgttggc ctggaactcg
      241 gtgtcgacgt gctgggtcgt atgccctaca aaggcagtgt ggaaaatggg gcgtataaag
      301 cacaagggta tcaagcaact cttaagatgg gcgtgccgat taccgatgac gtggacgtat
      361 atctgcgttt aggcggtatg gtatggcgtg cagataccaa atctaatgtt tatggcaaaa
      421 accatgatac tggcgttagc ccagtgatgg caggtggctc tgaatatgca attacccctg
      481 aaatcgcaat gcgcctcgaa acacaatgga ctaacaatat tggtgattac ccatacgatg
      541 ttccagatta cgctacccgt ccggacaatc tgatgctgtc tctgggtctg tcctaccgtt
      601 tcggttgttg ccctggttgc tgtcaccatc accaccatca ctaaaagctt ccccctagca
      661 taaccccttg gggcctctaa acgggtcttg aggggttttt tgcccctgag acgcgtcaat
      721 cgagttcgta cctaagggcg acaccccata attagcccgg gcgaaaggcc cagtctttcg
      781 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg
//
