LOCUS       sequence-114045-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 1256.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H1256
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     28..90
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             538..564
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             616..633
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             634..651
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      665..712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttgtttact tttaggagat aaaacatatg aaaaagaccg caatcgccat cgcagtggca
       61 ctggctggtt tcgctaccgt agcgcaggcc gcgccgaacg acaacacctg gtatactggt
      121 gctaaactgg gctggtccca gtaccacgac actggtttca tcaataataa tggcccgacc
      181 cacgagaacc agctgggcgc tggtgctttt ggtggttacc aggtgaaccc gtacgttggc
      241 ctggagctgg gcgttgacgt tctcggtcgt atgccgtaca aaggcagcgt tgaaaacggt
      301 gcatacaagg ctcagggcta tcaagcgacg ctgaaaatgg gtgtcccgat caccgacgac
      361 ctggatatct atactcgtct gggtggcatg gtatggcgtg cagatactaa atccaacgtt
      421 tatggtaaaa accacgatac cggcgtttct ccggtcttcg ctggcggtgt tgagtacgcg
      481 atcactccgg agatcgctat gcgtctggaa acgcaacaca ccaacaacat cggtgactac
      541 ccatacgatg ttccagatta cgcgactcgt ccggataact ggatggtgtc tctgggtgcg
      601 tcctaccgtt tcggttgttg ccctggttgc tgtcaccatc accaccatca ctaaaagctt
      661 ccccctagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgcccctgag
      721 acgcgtcaat cgagttcgta cctaagggcg acaccccata attagcccgg gcgaaaggcc
      781 cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctc
//
