LOCUS       sequence-11405-e        9131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Edwards SR, Wandless TJ
  TITLE     Dicistronic regulation of fluorescent proteins in the budding yeast 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2010 Apr;27(4):229-36. doi: 10.1002/yea.1744
  PUBMED    20017217
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea"; 
            journalName: "Yeast"; date: "2010-04"; volume: "27"; issue: "4"; 
            pages: "229-36"
COMMENT     Sequence Label: pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR
FEATURES             Location/Qualifiers
     source          1..9131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..719
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1973..2686
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1973..2686
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2696..3172
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     terminator      3559..3746
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3614..3638)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3863..4957)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4958..5363)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5405..5421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5405..5421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5418..5440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5429..5445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5453..5459,5460..5477,5478..5483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5498..5519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    5574..5607
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5685..5718
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5787..5804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5958..6546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6038..6057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6717..7508,7509..7577))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7340..7359
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7578..7682)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7750..7768
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7806..7828)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7928..7947
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      7964..9128
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggatcctt ttgttgtttc cgggtgtaca atatggactt cctcttttct
       61 ggcaaccaaa cccatacatc gggattccta taataccttc gttggtctcc ctaacatgta
      121 ggtggcggag gggagatata caatagaaca gataccagac aagacataat gggctaaaca
      181 agactacacc aattacactg cctcattgat ggtggtacat aacgaactaa tactgtagcc
      241 ctagacttga tagccatcat catatcgaag tttcactacc ctttttccat ttgccatcta
      301 ttgaagtaat aataggcgca tgcaacttct tttctttttt tttcttttct ctctcccccg
      361 ttgttgtctc accatatccg caatgacaaa aaaaatgatg gaagacacta aaggaaaaaa
      421 ttaacgacaa agacagcacc aacagatgtc gttgttccag agctgatgag gggtatctcg
      481 aagcacacga aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt
      541 atacggcctt ccttccagtt acttgaattt gaaataaaaa aagtttgctg tcttgctatc
      601 aagtataaat agacctgcaa ttattaatct tttgtttcct cgtcattgtt ctcgttccct
      661 ttcttccttg tttctttttc tgcacaatat ttcaagctat accaagcata caatcaactc
      721 cggtaccact agtatggtaa gtaagggtga agaagacaat atggcgatca ttaaggaatt
      781 catgcgtttc aaagtacaca tggagggaag cgtgaacgga catgaatttg aaatcgaagg
      841 ggaaggcgaa ggtagaccat acgaaggaac ccagaccgca aagcttaaag ttaccaaagg
      901 cgggccacta ccatttgcat gggatatctt gagccctcag tttatgtatg gcagtaaggc
      961 ctacgttaaa cacccagctg atattcccga ctatttgaaa ttgtcttttc cagaaggatt
     1021 caaatgggaa agagtaatga atttcgagga cggcggagtt gttactgtta ctcaagattc
     1081 aagtttgcaa gacggtgaat ttatttacaa ggtcaaatta agagggacta atttccctag
     1141 tgatggtccc gtcatgcaaa agaagactat gggttgggaa gcctcatctg aacgtatgta
     1201 tccagaagat ggcgcgctta agggggaaat taaacaaaga ttgaagttaa aagacggtgg
     1261 tcactacgac gcggaagtta agaccactta taaagctaaa aagcccgttc agttacctgg
     1321 tgcatataac gtaaacatta aattggatat cacttcacat aatgaagatt acactattgt
     1381 ggaacaatat gaaagagctg aaggtaggca ctcaacgggt ggaatggacg aattgtacaa
     1441 ataaccgccg gaaatcattt tttgaagatt acattaataa ggcttttttc aatatctctg
     1501 gaacaacagt ttttttttac ttactaatag ctttaaggac cctcttggac atcatgatgg
     1561 cagacttcca tcgtaatggg atgatcatat gatgggcgct atcctcatcg cgactcgata
     1621 acgacgtgag aaacgatttt ttttttcttt ttcaccgtat ttttgtgcgt cctttttcaa
     1681 ttatagcttt ttttttattt tttttttttc tcgtactgtt tcactgacaa aagttttttt
     1741 ttcaagaaaa attttcgatg ccgcgttctc tgtgtgcaac ggatggatgg tagatggaat
     1801 ttcaatatgt tgcttgaaat tttaccaatc ttgatattgt gataatttac ttaattatga
     1861 ttcttcctct tcccttcaat ttcttaaagc ttcttacttt actccttctt gctcataaat
     1921 aagcaaggta agaggacaac tgtaattacc tattacaata ccgcggggta ccatgtctaa
     1981 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
     2041 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac
     2101 cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
     2161 tttaacttat ggtgttcaat gtttttctag atacccagat catatgaaac aacatgactt
     2221 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
     2281 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
     2341 cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata
     2401 caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt
     2461 taacttcaaa attagacaca acattgaaga tggttctgtt caattagctg accattatca
     2521 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac
     2581 tcaatctgcc ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt
     2641 tgttactgct gctggtatta cccatggtat ggatgaattg tacaaagaga tctcaatcag
     2701 tctgattgcg gcgttagcgg tagatcacgt tatcggcatg gaaaacgcca tgccgtggaa
     2761 cctgcctgcc gatctcgcct ggtttaaacg caacacctta aataaacccg tgattatggg
     2821 ccgccatacc tgggaatcaa tcggtcgtcc gttgccagga cgcaaaaata ttatcctcag
     2881 cagtcaaccg agtacggacg atcgcgtaac gtgggtgaag tcggtggatg aagccatcgc
     2941 ggcgtgtggt gacgtaccag aaatcatggt gattggcggc ggtcgcgttt atgaacagtt
     3001 cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgac gcagaagtgg aaggcgacac
     3061 ccatttcccg gattacgagc cggatgactg ggaatcggta ttcagcgaat tccacgatgc
     3121 tgatgcgcag aactctcaca gctattgctt tgagattctg gagcggcgat aactcgagct
     3181 gcagatgaat cgtagatact gaaaaacccc gcaagttcac ttcaactgtg catcgtgcac
     3241 catctcaatt tctttcattt atacatcgtt ttgccttctt ttatgtaact atactcctct
     3301 aagtttcaat cttggccatg taacctctga tctatagaat tttttaaatg actagaatta
     3361 atgcccatct tttttttgga cctaaattct tcatgaaaat atattacgag ggcttattca
     3421 gaagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg
     3481 tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt
     3541 gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
     3601 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     3661 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
     3721 gctcttattg accacacctc taccggccgg tcgaaattcc cctaccctat gaacatattc
     3781 cattttgtaa tttcgtgtcg tttctattat gaatttcatt tataaagttt atgtacaaat
     3841 atcataaaaa aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat
     3901 caccgacttc ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca
     3961 aaaccttttt aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt
     4021 tcaacatcat tgcagcagac aagatagtgg cgatagggtt gaccttattc tttggcaaat
     4081 ctggagcaga accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag
     4141 aggccaagga cgcagatggc aacaaaccca aggaacctgg gataacggag gcttcatcgg
     4201 agatgatatc accaaacatg ttgctggtga ttataatacc atttaggtgg gttgggttct
     4261 taactaggat catggcggca gaatcaatca attgatgttg aaccttcaat gtaggaaatt
     4321 cgttcttgat ggtttcctcc acagtttttc tccataatct tgaagaggcc aaaacattag
     4381 ctttatccaa ggaccaaata ggcaatggtg gctcatgttg tagggccatg aaagcggcca
     4441 ttcttgtgat tctttgcact tctggaacgg tgtattgttc actatcccaa gcgacaccat
     4501 caccatcgtc ttcctttctc ttaccaaagt aaatacctcc cactaattct ctgacaacaa
     4561 cgaagtcagt acctttagca aattgtggct tgattggaga taagtctaaa agagagtcgg
     4621 atgcaaagtt acatggtctt aagttggcgt acaattgaag ttctttacgg atttttagta
     4681 aaccttgttc aggtctaaca ctacctgtac cccatttagg accacccaca gcacctaaca
     4741 aaacggcatc aaccttcttg gaggcttcca gcgcctcatc tggaagtggg acacctgtag
     4801 cgtcgatagc agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa
     4861 catcagaaat agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt
     4921 cacctggcaa aacgacgatc ttcttagggg cagacattag aatggtatat ccttgaaata
     4981 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa
     5041 ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat
     5101 gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc cggcgctctc
     5161 acctttcctt tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag gcgacctctg
     5221 aaattaacaa aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc ccctgtgtgt
     5281 tctcgttatg ttgaggaaaa aaataatggt tgctaagaga ttcgaactct tgcatcttac
     5341 gatacctgag tattcccaca gttggggatc tcgactctag ctagaggatc aattcgtaat
     5401 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5461 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5521 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgataactt
     5581 cgtataatgt atgctatacg aagttattag gtctgaagag gagtttacgt ccagccaagc
     5641 tagcttggct gcaggtcgag cggccgcgat ccggaaccct taatataact tcgtataatg
     5701 tatgctatac gaagttatca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5761 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5821 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5881 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5941 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6001 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6061 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6121 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6181 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6241 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6301 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6361 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     6421 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6481 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6541 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6601 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6661 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6721 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6781 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6841 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6901 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6961 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7021 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7081 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7141 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7201 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7261 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7321 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7381 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7441 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7501 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7561 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7621 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7681 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     7741 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     7801 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     7861 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     7921 aactatgcgg catcagagca gattgtactg agagtgcacc ataacgcatt taagcataaa
     7981 cacgcactat gccgttcttc tcatgtatat atatatacag gcaacacgca gatataggtg
     8041 cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt
     8101 tcggaaacgc tttgaagttc ctattccgaa gttcctattc tctagctaga aagtatagga
     8161 acttcagagc gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa
     8221 acgcaccgga ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct
     8281 caaaagtatc tctttgctat atatctctgt gctatatccc tatataacct acccatccac
     8341 ctttcgctcc ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag
     8401 tattactctt tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa
     8461 tacgaaaatg taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc
     8521 ataattttct gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg
     8581 caatccacat cggtatagaa tataatcggg gatgccttta tcttgaaaaa atgcacccgc
     8641 agcttcgcta gtaatcagta aacgcgggaa gtggagtcag gcttttttta tggaagagaa
     8701 aatagacacc aaagtagcct tcttctaacc ttaacggacc tacagtgcaa aaagttatca
     8761 agagactgca ttatagagcg cacaaaggag aaaaaaagta atctaagatg ctttgttaga
     8821 aaaatagcgc tctcgggatg catttttgta gaacaaaaaa gaagtataga ttctttgttg
     8881 gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt
     8941 ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca aaaatgaagc acagattctt
     9001 cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg taaaaatgca gctcagattc
     9061 tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ctacaaaatg aagcacagat
     9121 gcttcgttgc t
//
