LOCUS       sequence-114050-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 2368.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H2368
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     29..91
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             539..565
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             617..634
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             635..652
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      666..713
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttac ttttaggaga taaaacatat gaaaaagacc gcaatcgcca tcgcagtggc
       61 actggctggt ttcgctaccg tagcgcaggc cgcgccgaac gacaacacct tttacctggg
      121 tttgaagatg ggtatttccc agtaccacga cactggtttc atcaataata atggcccgac
      181 ccacgagaac cagctgggcg ctggtgcttt tggtggttac caggtgaacc cgtacgttgg
      241 ctttgaaatg ggttacgact ggctcggtcg tatgccgtac aaaggcagcg ttgaaaacgg
      301 tgcatacaag gctcagggct atcaagcgac gctgaaaatg ggtgtcccga tcaccgacga
      361 cgttgacgtg tacctgcgtc tgggcatcat ggtatggcgt gcagatacta aatccaacgt
      421 ttatggtaaa aaccacgata ccggcgtttc tccggtcttc gctggcggtg ttgagtacgc
      481 gatcactccg gagatcgcta tgcgtctgga aacgcaacac accaacaaca tcggtgacta
      541 cccatacgat gttccagatt acgcgactcg tccggataac ggcatgctga gcctgggtgt
      601 ttcctaccgt ttcggttgtt gccctggttg ctgtcaccat caccaccatc actaaaagct
      661 tccccctagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgcccctga
      721 gacgcgtcaa tcgagttcgt acctaagggc gacaccccat aattagcccg ggcgaaaggc
      781 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttcccc tactctcgca
      841 tggggagtc
//
