LOCUS       sequence-114051-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: Constitutive promoter - Anderson series - low 
            strength [E:J23103:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: J23103_EB
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..64)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    67..88
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        107..141
                     /label=BBa_J23112
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0"
     promoter        107..141
                     /label=BBa_J23103
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
     misc_feature    153..173
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(179..196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(430..449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
       61 tttctggaat tcgcggccgc ttctagagac tagtgggtct cagcttctga tagctagctc
      121 agtcctaggg attatgctag ctactagaga cctactagta gcggccgctg caggcttcct
      181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
      361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      781 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaa
//
