LOCUS       sequence-114054-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Wild-type OmpA with aromatic girdle residues replaced by leucine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_WTnoGirdles
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     28..90
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             550..576
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             631..648
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             649..666
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      680..727
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttgtttact tttaggagat aaaacatatg aaaaagaccg caatcgccat cgcagtggca
       61 ctggctggtt tcgctaccgt agcgcaggcc gcgccgaacg acaacacctt gtatactggt
      121 gctaaactgg gcttgtccca gtaccatgat actggtttca tcaataataa tggcccgacc
      181 catgaaaacc aactgggcgc tggtgctttt ggtggtttgc aggttaaccc gtatgttggc
      241 ttggaaatgg gtttggactt gttaggtcgt atgccgtata aaggcagcgt tgaaaacggt
      301 gcatataaag ctcagggcgt tcaactgacc gctaaactgg gtttgccaat cactgacgac
      361 ctggatatct atactcgtct gggtggcatg gtatggcgtg cagatactaa atccaacgtt
      421 tatggtaaaa accacgatac cggcgtttct ccggtcttgg ctggcggtgt tgagttggcg
      481 atcactcctg aaatcgctac ccgtctggaa ttgcagttga ccaataatat cggtgacgcg
      541 cataccatct acccatacga tgttccagat tacgctggca ctcgtccgga taacggcatg
      601 ctgagcctgg gtgtttccta ccgtttcggt tgttgccctg gttgctgtca ccatcaccac
      661 catcactaaa agcttccccc tagcataacc ccttggggcc tctaaacggg tcttgagggg
      721 ttttttgccc ctgagacgcg tcaatcgagt tcgtacctaa gggcgacacc ccataattag
      781 cccgggcgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt
      841 ccctactctc gcatggggag tccccacact accatcggcg
//
