LOCUS       sequence-114057-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 1237.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H1237
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     27..89
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             537..563
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             615..632
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             633..650
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      664..711
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttgtttactt ttaggagata aaacatatga aaaagaccgc aatcgccatc gcagtggcac
       61 tggctggttt cgctaccgta gcgcaggccg cgccgaacga caacacctgg tatactggtg
      121 ctaaactggg ctggtcccag taccacgaca ctggtttcat caataataat ggcccgaccc
      181 acgagaacca gctgggcgct ggtgcttttg gtggttacca ggtgaacccg tacgttggct
      241 ttgaaatggg ttacgactgg ctcggtcgta tgccgtacaa aggcagcgtt gaaaacggtg
      301 catacaaggc tcagggctat caagcgacgc tgaaaatggg tgtcccgatc accgacgacg
      361 ttgacgtgta cctgcgtctg ggcatcatgg tatggcgtgc agatactaaa tccaacgttt
      421 atggtaaaaa ccacgatacc ggcgtttctt tcgtcatggc gctgggtagc gaacatgcga
      481 tcactccgga gatcgctacc cgtctggaat accagtggac caacaacatc ggtgactacc
      541 catacgatgt tccagattac gcgactcgtc cggataactg gatggtgtct ctgggtgcgt
      601 cctaccgttt cggttgttgc cctggttgct gtcaccatca ccaccatcac taaaagcttc
      661 cccctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gcccctgaga
      721 cgcgtcaatc gagttcgtac ctaagggcga caccccataa ttagcccggg cgaaaggccc
      781 agtctttcga ctgagccttt cgttttattt tgatgcctgg cagttcccta ctctcgcatg
//
