LOCUS       sequence-114067-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 4568.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H4568
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     26..88
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             536..562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             614..631
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             632..649
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      663..710
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgtttacttt taggagataa aacatatgaa aaagaccgca atcgccatcg cagtggcact
       61 ggctggtttc gctaccgtag cgcaggccgc gccgaacgac aacacctttt acctgggttt
      121 gaagatgggt atttcccagt accacgacac tggtttcatc aataataatg gcccgaccca
      181 cgagaaccag cagggtatcg gtgcctttgc aggcatccag gtgaacccgt acgttggcct
      241 ggagctgggc gttgacgttc tcggtcgtat gccgtacaaa ggcagcgttg aaaacggtgc
      301 atacaaggct cagggcgttc aactgaccgc taaactgggt tacccgatca ccgacgacct
      361 ggatatctat actcgtctgg gtggcatggt atggcgtgca gatactaaat ccaacgttta
      421 tggtaaaaac cacgataccg gcgtttctcc ggtcttcgct ggcggtgttg agtacgcgat
      481 cactccggag atcgctatgc gtctggaaac gcaacacacc aacaacatcg gtgactaccc
      541 atacgatgtt ccagattacg cgactcgtcc ggataacggc atgctgagcc tgggtgtttc
      601 ctaccgtttc ggttgttgcc ctggttgctg tcaccatcac caccatcact aaaagcttcc
      661 ccctagcata accccttggg gcctctaaac gggtcttgag gggttttttg cccctgagac
      721 gcgtcaatcg agttcgtacc taagggcgac accccataat tagcccgggc gaaaggccca
      781 gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg
      841 gagtcccc
//
