LOCUS       sequence-11407-e       10481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T
  TITLE     GSK3beta phosphorylation modulates CLASP-microtubule association and
            lamella microtubule attachment.
  JOURNAL   J Cell Biol. 2009 Mar 23. 184(6):895-908.
  PUBMED    19289791
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Mar 23. 184(6):895-908."
COMMENT     Sequence Label: pShuttle-EGFP-CLASP2 340-1084 9xS/A
FEATURES             Location/Qualifiers
     source          1..10481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        399..702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        703..906
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     857..877
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      946..955
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(952..954,955..957,958..1668)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(952..954,955..957,958..1668)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(997..1018)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1258..1277)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1605..1626
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4129..4250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4166..4185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4220..4239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7441..7543
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7632..7649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7803..8391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7883..7902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9223..10017
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9223..10017
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9277..9296)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9887..9906
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(10065..10444)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10127..10148)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10339..10358
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      901 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgccac catggtgagc
      961 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1021 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1081 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1141 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1201 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1261 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1321 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1381 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1441 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1501 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1561 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1621 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ctcagatctc
     1681 gagctcaagc ttcgaattca tgatgctgac gctgaggcca gagtggaggc aagaaagaca
     1741 tacatgggtc ttagaaacca ctttcctggt gaagctgaaa cattatataa ttcccttgag
     1801 ccatcttatc agaagagtct tcaaacttac ttaaagagtt ctggcagtgt agcatctctt
     1861 ccacaatcag acaggtcctc atccagctca caggaaagtc tcaatcgccc tttttcttcc
     1921 aaatggtcta cagcaaatcc atcaactgtg gctggaagag tatcagcagg cagcagcaaa
     1981 gccagttccc ttccaggaag cctgcagcgt tcacgaagtg acattgatgt gaatgctgct
     2041 gcaggtgcca aggcacatca tgctgctgga cagtctgtgc gaagggggcg cttaggtgca
     2101 ggtgccctga atgcaggttc ctatgcgtca ctagaggata cttctgacaa gctggatgga
     2161 acagcatctg aagatggccg ggtgagagca aaactttcag caccacttgc tggcatggga
     2221 aatgccaagg cagattctag aggaagaagt cgaacaaaaa tggtgtctca atcacagcct
     2281 ggtagccggt ctgggtctcc aggaagagtt ctgaccacaa cagccctgtc cactgtgagc
     2341 tctggtgttc aaagagtcct ggtcaattca gcctcagcac aaaaaagaag caagatacca
     2401 cgggcccagg gctgtgccag agaggctgct ccatctaggc tttcagtggc ccgaagcagt
     2461 cgtattcctc gaccaagtgt ggctcaagga tgcgcccggg aagctgctcg ggagagcgcc
     2521 agagacacag ctcctgttcg ctcttttcag cccctcgcct ccagacacca ttccagatca
     2581 actggtgccc tctacgcccc cgaagtgtat ggggcctcag gtccaggtta tgggatcagc
     2641 caatcaagtc gactgtcgtc ttctgttagt gccatgcgag tcctgaacac aggttctgat
     2701 gtggaggagg cggtggcaga tgccttgaaa aaaccagctc gaagaagata tgaatcatat
     2761 ggaatgcatt cagatgatga cgccaacagc gatgcatcta gtgcttgttc agaacgctcc
     2821 tatagttctc gaaatggtag tattcctaca tatatgaggc agacggaaga tgtggcagaa
     2881 gtcctcaata gatgtgctag ttccaattgg tcagaaagga aagaaggcct cctaggtctg
     2941 cagaacttat taaaaaatca gagaacacta agtcgagttg aactgaaaag attatgtgaa
     3001 attttcacaa gaatgtttgc tgaccctcat ggcaagagag tattcagcat gtttttggag
     3061 actctagtgg atttcataca agtccacaaa gatgatcttc aagattggtt gtttgtactg
     3121 ctgacacaac tactaaaaaa aatgggtgct gatttgcttg gatctgttca ggcaaaagtt
     3181 cagaaagccc ttgatgttac aagagagtct tttccaaatg atcttcagtt caatattcta
     3241 atgagattta cagttgatca gacccagaca ccaagcttaa aggtgaaggt tgctatcctt
     3301 aaatacatag aaactctggc caaacagatg gatccaggag attttataaa ttccagtgaa
     3361 actcgcctag cagtgtctcg ggtcatcact tggacaacag aacccaaaag ttctgatgtt
     3421 cggaaggcag cacagtcagt gctgatttca ttatttgaac tcaatacccc agagtttaca
     3481 atgttattag gagctttacc aaaaactttt caggatggtg ctaccaagct tcttcataat
     3541 caccttcgaa acactggcaa tggaacccag agttccatgg ggagtccttt gacaagacca
     3601 acaccacgat caccagctaa ctggtccagt cctcttactt ctcctaccaa tacatcacag
     3661 aatactttat ctccaagtgc atttgattat gacacagaaa atatgaactc tgaagatatt
     3721 tatagctctc ttagaggtgt cactgaagca atccagaatt tcagcttccg tagccaagaa
     3781 gatatgaatg agccattgaa aagggattct aaaaaagatg atggcgattc aatgtgtggt
     3841 ggtcctggga tgtctgaccc aagagcagga ggtgatgcta ctgactcaag tcaaacagct
     3901 cttgataata aagcttcatt gctccattca atgcctactc actcctctcc acgctctcga
     3961 taggcggccg ctcgagccta agcttctaga taagatatcc gatccaccgg atctagataa
     4021 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     4081 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4141 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4201 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcggatct
     4261 gggcgtggtt aagggtggga aagaatatat aaggtggggg tcttatgtag ttttgtatct
     4321 gttttgcagc agccgccgcc gccatgagca ccaactcgtt tgatggaagc attgtgagct
     4381 catatttgac aacgcgcatg cccccatggg ccggggtgcg tcagaatgtg atgggctcca
     4441 gcattgatgg tcgccccgtc ctgcccgcaa actctactac cttgacctac gagaccgtgt
     4501 ctggaacgcc gttggagact gcagcctccg ccgccgcttc agccgctgca gccaccgccc
     4561 gcgggattgt gactgacttt gctttcctga gcccgcttgc aagcagtgca gcttcccgtt
     4621 catccgcccg cgatgacaag ttgacggctc ttttggcaca attggattct ttgacccggg
     4681 aacttaatgt cgtttctcag cagctgttgg atctgcgcca gcaggtttct gccctgaagg
     4741 cttcctcccc tcccaatgcg gtttaaaaca taaataaaaa accagactct gtttggattt
     4801 ggatcaagca agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg taggcccggg
     4861 accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg taaaggtgac
     4921 tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag caccactgca
     4981 gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag cgctgggcgt
     5041 ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc ttggtgtaag
     5101 tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga tgcatcttgg
     5161 actgtatttt taggttggct atgttcccag ccatatccct ccggggattc atgttgtgca
     5221 gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc ttagaaggaa
     5281 atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg cattcgtcca
     5341 taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg ggatcactaa
     5401 cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag cgcgggcgga
     5461 gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta ccctcacaga
     5521 tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc ggggcgatga
     5581 agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc ctgagcagct
     5641 gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccggctgc aactggtagt
     5701 taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta agcatgtccc
     5761 tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc agcgatagca
     5821 gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta ggcatgcttt
     5881 tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc tctacggcat
     5941 ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt acggcagtag
     6001 tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg tcctcgtcag
     6061 cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca gggtgcgctt
     6121 gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt cggccaggta
     6181 gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg cgcgcagctt
     6241 gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt agagcttggg
     6301 cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc agacggtctc
     6361 gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt ttcccccatg
     6421 ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct cggtgacgaa
     6481 aaggctgtcc gtgtccccgt atacagactt gagagggagt ttaaacgaat tcaatagctt
     6541 gttgcatggg cggcgatata aaatgcaagg tgctgctcaa aaaatcaggc aaagcctcgc
     6601 gcaaaaaaga aagcacatcg tagtcatgct catgcagata aaggcaggta agctccggaa
     6661 ccaccacaga aaaagacacc atttttctct caaacatgtc tgcgggtttc tgcataaaca
     6721 caaaataaaa taacaaaaaa acatttaaac attagaagcc tgtcttacaa caggaaaaac
     6781 aacccttata agcataagac ggactacggc catgccggcg tgaccgtaaa aaaactggtc
     6841 accgtgatta aaaagcacca ccgacagctc ctcggtcatg tccggagtca taatgtaaga
     6901 ctcggtaaac acatcaggtt gattcacatc ggtcagtgct aaaaagcgac cgaaatagcc
     6961 cgggggaata catacccgca ggcgtagaga caacattaca gcccccatag gaggtataac
     7021 aaaattaata ggagagaaaa acacataaac acctgaaaaa ccctcctgcc taggcaaaat
     7081 agcaccctcc cgctccagaa caacatacag cgcttccaca gcggcagcca taacagtcag
     7141 ccttaccagt aaaaaagaaa acctattaaa aaaacaccac tcgacacggc accagctcaa
     7201 tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta tatataggac taaaaaatga
     7261 cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc acgcgaacct acgcccagaa
     7321 acgaaagcca aaaaacccac aacttcctca aatcgtcact tccgttttcc cacgttacgt
     7381 cacttcccat tttaagaaaa ctacaattcc caacacatac aagttactcc gccctaaaac
     7441 ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac cccctcatta
     7501 tcatattggc ttcaatccaa aataaggtat attattgatg atgttaatta acatgcatgg
     7561 atccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     7621 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7681 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7741 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7801 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7861 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7921 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7981 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8041 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8101 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8161 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8221 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8281 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8341 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8401 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8461 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8521 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8581 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8641 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8701 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8761 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8821 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     8881 gccatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg
     8941 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac
     9001 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg
     9061 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
     9121 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     9181 ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     9241 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     9301 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     9361 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     9421 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     9481 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     9541 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     9601 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     9661 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     9721 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     9781 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     9841 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     9901 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     9961 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt
    10021 ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaccat
    10081 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
    10141 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
    10201 cgatggccca ctacgtgaac catcacccta atcaagtttt ttgtggtcga ggtgccgtaa
    10261 agatctaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
    10321 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
    10381 tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc
    10441 gcgtccattc gccattcagg atcgaattaa ttcttaatta a
//
