LOCUS       sequence-114071-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conservative version of OmpA Redesign 4 with G99I mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_R4cons_G99I
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     17..79
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             593..610
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             611..628
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      642..689
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttaggagata aaacatatga aaaagaccgc aatcgccatc gcagtggcac tggctggttt
       61 cgctaccgta gcgcaggccg cgccgaaaga caacaccttc tatctcggcc tgaaaatggg
      121 gatcagccag tatcacgata cgggatttat taacaataat ggtccgacgc atgaaaatca
      181 gctggggatc ggcgcatttg gaggtatcca ggtcaatccc tacgttggcc tggaactcgg
      241 tgtcgacgtg ctgggtcgta tgccctacaa aggcagtgtg gaaaatgggg cgtataaagc
      301 acaagggtat caagcaactc ttaagatggg cgtgccgatt accgatgacg tggacgtata
      361 tctgcgttta ggcatcatgg tatggcgtgc agataccaaa tctaatgttt atggcaaaaa
      421 ccatgatact ggcgttagcc cagtgatggc aggtggctct gaatatgcaa ttacccctga
      481 aatcgcaatg cgcctcgaaa cacaatggac taacaatatt ggtgatgcgc acaccattgg
      541 tacccgtccg gacaatctga tgctgtctct gggtctgtcc taccgtttcg gttgttgccc
      601 tggttgctgt caccatcacc accatcacta aaagcttccc cctagcataa ccccttgggg
      661 cctctaaacg ggtcttgagg ggttttttgc ccctgagacg cgtcaatcga gttcgtacct
      721 aagggcgaca ccccataatt agcccgggcg aaaggcccag tctttcgact gagcctttcg
      781 ttttatttga tgcctggcag ttcccctact ctcgcatggg gagtccccac actaccatc
//
