LOCUS       sequence-114075-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 123578.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H-46
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     16..78
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             526..552
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             604..621
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             622..639
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      653..700
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taggagataa aacatatgaa aaagaccgca atcgccatcg cagtggcact ggctggtttc
       61 gctaccgtag cgcaggccgc gccgaacgac aacacctggt atactggtgc taaactgggc
      121 tggtcccagt accacgacac tggtttcatc aataataatg gcccgaccca cgagaaccag
      181 ctgggcgctg gtgcttttgg tggttaccag gtgaacccgt acgttggctt tgaaatgggt
      241 tacgactggc tcggtcgtat gccgtacaaa ggcagcgttg aaaacggtgc atacaaggct
      301 cagggctatc aagcgacgct gaaaatgggt gtcccgatca ccgacgacct ggatatctat
      361 actcgtctgg gtggcatggt atggcgtgca gatactaaat ccaacgttta tggtaaaaac
      421 cacgataccg gcgtttcttt cgtcatggcg ctgggtagcg aacatgcgat cactccggag
      481 atcgctaccc gtctggaata ccagtggacc aacaacatcg gtgactaccc atacgatgtt
      541 ccagattacg cgactcgtcc ggataacggc atgctgagcc tgggtgtttc ctaccgtttc
      601 ggttgttgcc ctggttgctg tcaccatcac caccatcact aaaagcttcc ccctagcata
      661 accccttggg gcctctaaac gggtcttgag gggttttttg cccctgagac gcgtcaatcg
      721 agttcgtacc taagggcgac accccataat tagccccggg cgaaaggccc agtctttcga
      781 ctgagccttt cgttt
//
