LOCUS       sequence-114076-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 346.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H346
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     17..79
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             527..553
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             605..622
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             623..640
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      654..701
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttaggagata aaacatatga aaaagaccgc aatcgccatc gcagtggcac tggctggttt
       61 cgctaccgta gcgcaggccg cgccgaacga caacaccttt tacctgggtt tgaagatggg
      121 tatttcccag taccacgaca ctggtttcat caataataat ggcccgaccc acgagaacca
      181 gcagggtatc ggtgcctttg caggcatcca ggtgaacccg tacgttggct ttgaaatggg
      241 ttacgactgg ctcggtcgta tgccgtacaa aggcagcgtt gaaaacggtg catacaaggc
      301 tcagggcgtt caactgaccg ctaaactggg ttacccgatc accgacgacg ttgacgtgta
      361 cctgcgtctg ggcatcatgg tatggcgtgc agatactaaa tccaacgttt atggtaaaaa
      421 ccacgatacc ggcgtttctc cggtcttcgc tggcggtgtt gagtacgcga tcactccgga
      481 gatcgctatg cgtctggaaa cgcaacacac caacaacatc ggtgactacc catacgatgt
      541 tccagattac gcgactcgtc cggataactg gatggtgtct ctgggtgcgt cctaccgttt
      601 cggttgttgc cctggttgct gtcaccatca ccaccatcac taaaagcttc cccctagcat
      661 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gcccctgaga cgcgtcaatc
      721 gagttcgtac ctaagggcga caccccataa ttagcccggg cgaaaggccc agtctttcga
      781 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg ggagtcccca
      841 cactaccat
//
