LOCUS       sequence-114077-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 123456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H-78
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     14..76
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             524..550
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             602..619
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             620..637
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      651..698
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggagataaaa catatgaaaa agaccgcaat cgccatcgca gtggcactgg ctggtttcgc
       61 taccgtagcg caggccgcgc cgaacgacaa cacctggtat actggtgcta aactgggctg
      121 gtcccagtac catgatactg gtttcatcaa taataatggc ccgacccatg aaaaccaact
      181 gggcgctggt gcttttggtg gttaccaggt taacccgtat gttggctttg aaatgggtta
      241 cgactggtta ggtcgtatgc cgtataaagg cagcgttgaa aacggtgcat ataaagctca
      301 gggcgttcaa ctgaccgcta aactgggtta cccaatcact gacgacctgg atatctatac
      361 tcgtctgggt ggcatggtat ggcgtgcaga tactaaatcc aacgtttatg gtaaaaacca
      421 cgataccggc gtttctccgg tcttcgctgg cggtgttgag tacgcgatca ctccggagat
      481 cgctatgcgt ctggaaacgc aacacaccaa caacatcggt gactacccat acgatgttcc
      541 agattacgcg actcgtccgg ataactggat ggtgtctctg ggtgcgtcct accgtttcgg
      601 ttgttgccct ggttgctgtc accatcacca ccatcactaa aagcttcccc ctagcataac
      661 cccttggggc ctctaaacgg gtcttgaggg gttttttgcc cctgagacgc gtcaatcgag
      721 ttcgtaccta agggcgacac cccataatta gcccgggcga aaggcccagt ctttcgactg
      781 agcctttcgt ttatttg
//
