LOCUS       sequence-114113-        9011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for GATA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Elcheva I, Brok-Volchanskaya V, Kumar A, Liu P, Lee JH, Tong L, 
            Vodyanik M, Swanson S, Stewart R, Kyba M, Yakubov E, Cooke J, 
            Thomson JA, Slukvin I
  TITLE     Direct induction of haematoendothelial programs in human pluripotent
            stem cells by transcriptional regulators.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4372. doi: 10.1038/ncomms5372.
  PUBMED    25019369
REFERENCE   2  (bases 1 to 9011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4372. doi"; pages: " 
            10.1038/ncomms5372"
COMMENT     Sequence Label: pSIN4-EF1a-GATA2-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..9011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1415..1648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1833..1877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2164..2281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2410..3588
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2641..3579
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3536..3556
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3606..3632
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(5127..5701,5702..5704,5705..5713)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5294..5311)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5521..5540
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5733..6332
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5733..5752)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6229..6249
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6535..6768
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6919..6936)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6925..7149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      7192..7201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7245..7349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7350..7418,7419..8210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7568..7587)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8381..8969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8870..8889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg
     2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     2401 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2461 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2521 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2581 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2641 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     2701 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     2761 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     2821 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     2881 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     2941 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     3001 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     3061 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     3121 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     3181 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     3241 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     3301 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     3361 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     3421 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     3481 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     3541 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg gaattcgcca
     3601 ccatgtaccc atacgatgtt ccagattacg ctgaggtggc gccggagcag ccgcgctgga
     3661 tggcgcaccc ggccgtgctg aatgcgcagc accccgactc acaccacccg ggcctggcgc
     3721 acaactacat ggaacccgcg cagctgctgc ctccagacga ggtggacgtc ttcttcaatc
     3781 acctcgactc gcagggcaac ccctactatg ccaaccccgc tcacgcgcgg gcgcgcgtct
     3841 cctacagccc cgcgcacgcc cgcctgaccg gaggccagat gtgccgccca cacttgttgc
     3901 acagcccggg tttgccctgg ctggacgggg gcaaagcagc cctctctgcc gctgcggccc
     3961 accaccacaa cccctggacc gtgagcccct tctccaagac gccactgcac ccctcagctg
     4021 ctggaggccc tggaggccca ctctctgtgt acccaggggc tgggggtggg agcgggggag
     4081 gcagcgggag ctcagtggcc tccctcaccc ctacagcagc ccactctggc tcccaccttt
     4141 tcggcttccc acccacgcca cccaaagaag tgtctcctga ccctagcacc acgggggctg
     4201 cgtctccagc ctcatcttcc gcggggggta gtgcagcccg aggagaggac aaggacggcg
     4261 tcaagtacca ggtgtcactg acggagagca tgaagatgga aagtggcagt cccctgcgcc
     4321 caggcctagc tactatgggc acccagcctg ctacacacca ccccatcccc acctacccct
     4381 cctatgtgcc ggcggctgcc cacgactaca gcagcggact cttccacccc ggaggcttcc
     4441 tggggggacc ggcctccagc ttcaccccta agcagcgcag caaggctcgt tcctgttcag
     4501 aaggccggga gtgtgtcaac tgtggggcca cagccacccc tctctggcgg cgggacggca
     4561 ccggccacta cctgtgcaat gcctgtggcc tctaccacaa gatgaatggg cagaaccgac
     4621 cactcatcaa gcccaagcga agactgtcgg ccgccagaag agccggcacc tgttgtgcaa
     4681 attgtcagac gacaaccacc accttatggc gccgaaacgc caacggggac cctgtctgca
     4741 acgcctgtgg cctctactac aagctgcaca atgttaacag gccactgacc atgaagaagg
     4801 aagggatcca gactcggaac cggaagatgt ccaacaagtc caagaagagc aagaaagggg
     4861 cggagtgctt cgaggagctg tcaaagtgca tgcaggagaa gtcatccccc ttcagtgcag
     4921 ctgccctggc tggacacatg gcacctgtgg gccacctccc gcccttcagc cactccggac
     4981 acatcctgcc cactccgacg cccatccacc cctcctccag cctctccttc ggccaccccc
     5041 acccgtccag catggtgacc gccatgggct agacgcgtcg gaaactagtt cgaaggatcc
     5101 gcatgcatct agggcggcca attccgcccc tctccctccc ccccccctaa cgttactggc
     5161 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgtgattttc caccatattg
     5221 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5281 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5341 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5401 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5461 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5521 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     5581 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     5641 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     5701 gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc
     5761 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     5821 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     5881 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     5941 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6001 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6061 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6121 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6181 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6241 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga
     6301 cctggtgcat gacccgcaag cccggtgcct gataataggc ggccgctcga gacctagaaa
     6361 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6421 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6481 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6541 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6601 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6661 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6721 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6781 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     6841 cttgacatta taatagattt agcaggaatt gaactaggag tggagcacac aggcaaagtt
     6901 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6961 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7021 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7081 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7141 gctctatggc ttctgaggcg gaaagaacca gctgggggcg cgcccctcga ggccgccatg
     7201 gtcatagctg tttgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8521 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8761 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9001 tggccttttg c
//
