LOCUS       sequence-114123-        2162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: Controllable promoter - pTet - tetR regulated 
            (strong constitutive promoter repressed by tetR, C0040, which can 
            itself be inhibited by tetracycline or aTc) [E:R0040:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: R0040 (pTet)_EB
FEATURES             Location/Qualifiers
     source          1..2162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5..23
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    30..48
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    70..90
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(96..113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(267..855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(347..366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1026..1817,1818..1886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1649..1668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1887..1991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2059..2077
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2081..2124)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2127..2148
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gctttcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta
       61 ctagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg
      121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      241 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac
      301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1141 ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga tttatcagca
     1201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1321 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1621 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2041 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
     2101 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac tagtgggtct
     2161 ca
//
