LOCUS       sequence-114126-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: Controllable promoter - pTet - tetR regulated 
            (strong constitutive promoter repressed by tetR, C0040, which can 
            itself be inhibited by tetracycline or aTc) [G:R0040:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: R0040 (pTet)_GB
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..89
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    133..151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    173..193
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(199..216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(450..469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcatgcctcc ctatcagtga
      121 tagagattga catccctatc agtgatagag atactgagca ctactagaga cctactagta
      181 gcggccgctg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      241 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      301 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      361 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      421 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      481 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      541 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      601 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      661 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      721 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      781 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      841 gccagttacc ttcggaaa
//
