LOCUS       sequence-114130-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated
            (right promoter, weak constitutive down regulated by LuxR, C0062 in 
            combination with homoserine lactone (HSL)) [E:R0063:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: R0063 (pLuxR-pR)_EB
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..89
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    270..290
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(296..313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(547..566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcagcttacc tgtacgatcc
      121 tacaggtgct tatgttaagt aattgtattc ccagcgatac aatagtgtga caaaaatcca
      181 atttattaga atcaaatgtc aatccattac cgttttaatg atatataaca cgcaaaactt
      241 gcgacaaaca ataggtaata ctagagacct actagtagcg gccgctgcag gcttcctcgc
      301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      421 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
      481 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      841 gagcgaggta tgtaggcggt gctacagagt tctt
//
