LOCUS       sequence-114136-         510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: Controllable promoter - pBAD - araC regulated 
            (pBAD promoter, inducible by expression of AraC, C0080) 
            [A:I13453:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: I13453 (pBAD)_AB
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..89
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     141..160
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    249..269
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(275..292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcaggagaca ttgattattt
      121 gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag cggatcctac
      181 ctgacgcttt ttatcgcaac tctctactgt ttctccatac cgtttttttg ggctagctac
      241 tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg ctgcgctcgg
      301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      421 gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca
      481 aaaatcgacg ctcaagtcag aggtggcgaa
//
