LOCUS       sequence-114139-        2238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: Controllable promoter - pBAD - araC regulated 
            (pBAD promoter, inducible by expression of AraC, C0080) 
            [E:I13453:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: I13453 (pBAD)_EB
FEATURES             Location/Qualifiers
     source          1..2238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..57
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    146..166
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(172..189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(343..931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(423..442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1102..1893,1894..1962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1725..1744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1963..2067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2135..2153
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2157..2200)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2203..2224
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcttacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
       61 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccataccgt
      121 ttttttgggc tagctactag agacctacta gtagcggccg ctgcaggctt cctcgctcac
      181 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      241 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      301 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
      361 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      421 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      481 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      541 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      601 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      661 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      721 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      781 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      841 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      901 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      961 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1021 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1081 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1141 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1201 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc
     1261 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1321 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1381 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1441 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1501 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1561 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1621 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1681 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1741 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1801 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1861 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1921 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1981 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2041 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     2101 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
     2161 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct
     2221 agagactagt gggtctca
//
