LOCUS       sequence-114145-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli reporter vector with MCS for inserting bacterial promoter 
            region through 5' end of gene of interest, upstream and in-frame 
            with reporter cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Cress BF, Toparlak OD, Guleria S, Lebovich M, Stieglitz JT, 
            Englaender JA, Jones JA, Linhardt RJ, Koffas MA
  TITLE     CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR 
            Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E.
            coli.
  JOURNAL   ACS Synth Biol. 2015 Mar 30.
  PUBMED    25822415
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Mar 30."
COMMENT     Sequence Label: pCRISPReporter
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcccattcg ccaatccgga tatantcgac gcttggattc tgcgtttgtt gctagcncta
       61 gtgagacgag acgagacagc ctcctttggt cgaaaaaaaa agcccgcact gtcaggtgcg
      121 ggcttttttc tgtgtttccg gtacccgacc ttgatgtttc cagtgcgatt gaggaccttc
      181 agtgccatat gtgaaccgcc accgccggaa ccgccaccgc cactaccgcc accgccctcg
      241 agcaattgag atctaagctt gaattcttaa ttaaagcggc cgcgcgatcg ccgagaatgg
      301 atgcgagtaa tgttgagaag agaaaagaaa accgccgatc ctgtccaccg cattactgca
      361 aggtagtgga caagaccggc ggtcttaagt tttttggctg aatctagacc taggtccgtc
      421 tacgaactcc cagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
      481 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
      541 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
      601 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
      661 ctgcgcgttg gtgcggacat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
      721 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
      781 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
      841 tcactggt
//
