LOCUS       sequence-114148-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli reporter vector with MCS for inserting bacterial promoter 
            region through 5' end of gene of interest, upstream and in-frame 
            with reporter cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Cress BF, Toparlak OD, Guleria S, Lebovich M, Stieglitz JT, 
            Englaender JA, Jones JA, Linhardt RJ, Koffas MA
  TITLE     CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR 
            Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E.
            coli.
  JOURNAL   ACS Synth Biol. 2015 Mar 30.
  PUBMED    25822415
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Mar 30."
COMMENT     Sequence Label: pCRISPReporter
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
       61 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
      121 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta
      181 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
      241 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
      301 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
      361 cgcagacgcg ccgagacaga acttaatggg cccgctggga gttcgtagac ggacctaggt
      421 ctagattcag ccaaaaaact taagaccgcc ggtcttgtcc actaccttgc agtaatgcgg
      481 tggacaggat cggcggtttt cttttctctt ctcaacatta ctcgcatcca ttctcggcga
      541 tcgcgcggcc gctttaatta agaattcaag cttagatctc aattgctcga gggcggtggc
      601 ggtagtggcg gtggcggttc cggcggtggc ggttcacata tggcactgaa ggtcctcaat
      661 cgcactggaa acatcaaggt cgggtaccgg aaacacagaa aaaagcccgc acctgacagt
      721 gcgggctttt tttttcgacc aaaggaggct gtctcgtctc gtctcactag tgctagcaac
      781 aaacgcagaa tccaagcgtc gactatatcc ggattggcga atgggacgcg ccctgtagcg
      841 gcgcattaag cgcg
//
