LOCUS       sequence-114157-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part 
            type, actually contains a small transcriptional unit and second RBS)
            [B:BCD2:C].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: BCD2_BC
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..89
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             178..186
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    203..223
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(229..246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(480..499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcatactggg cccaagttca
      121 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgctaa
      181 ggaggttttc taatgagaga cctactagta gcggccgctg caggcttcct cgctcactga
      241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc
      421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      841 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcg
//
