LOCUS       sequence-11416-e       20082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20082)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 20082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2
FEATURES             Location/Qualifiers
     source          1..20082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      771..780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             777..1580
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(840..859)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1450..1469
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1740..1861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1777..1796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1831..1850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    1882..6158
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        6208..6707
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6243..6266)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6640..6659
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6734..7306
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          7383..7448
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(7516..7533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7522..7746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7814..7834)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7814..7832)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7853..7869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7853..7869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7866..7888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7877..7893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7901..7907,7908..7925,7926..7931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7946..7967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8255..8843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9014..9805,9806..9874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9637..9656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9875..9979)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10005..10460)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10142..10163)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10354..10373
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10587..10609
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10601..10618
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10602..10618
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10628..10647
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10628..10646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    10664..11748
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     primer_bind     11852..11874
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     protein_bind    11922..11946
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11979..12012)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        12703..12914
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             12927..13195
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             join(13251..13871,13872..14258)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(13324..13343)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     intron          14295..14867
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(14999..15018)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    15064..15119
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(15118..15137)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(15958..15978)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    16005..16053
                     /note="synthetic polyadenylation signal"
     CDS             complement(17417..17473)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(17498..18205)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(17773..17792)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     18042..18060
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      18202..18211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          18284..18856
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(18969..18993)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(18990..19028)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(19061..19079,19080..19102,19103..19121,19122..19144,
                     19145..19163,19164..19186,19187..19205,19206..19228,
                     19229..19247,19248..19270,19271..19289,19290..19312,
                     19313..19331)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    complement(20017..20050)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(join(20072..20082,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatcg cgtgcatgcg acgtcatagc ttaattaagg cgcgccgatg
       61 gaccgattcc tcccgataag taaattacag aaaagctcag gttaaatgta agaggatctt
      121 aaatattgat ttggtttttg tcttaatggt gcccggcgta tttatacgaa ccattaaatt
      181 tcactagacc gtttgtagtc agtggtgaac gctgcatgat ccacgctctc aaagtgagtt
      241 gtgttgcttt gcgggaatcc agcagtcaca ttaatgctgg aaaaagttca ggcaactagt
      301 gtactttgta gcggggtccg ggagaggctg gggtctagag tggcgatggg acgaaagtgt
      361 aaagaaagtt ttcggtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtaggtag
      421 gtaggtagaa actgcatcct ctgcttgtgt aaattcggtt acaagtctgt aacagaggta
      481 ctgagatgtg actgagtctc aaggccaggc gatcgggatt ctgaagaggc atttgcataa
      541 ctgagcaaga gtctttgccc cacccttcca cgccgaccca agacaagggg acagctctgg
      601 gtgggtcacc tgaagggcca ggggcccgcc cacatccccg cccacaccca ccttgagcgc
      661 ttctcattgg gcattccagc ctacccagct cggagttagt tactccgtaa gtgtggccgg
      721 aacagagttc gtccatctaa aaagggaggg gaccggcgaa cacatctaga gccaccatgg
      781 gatcggccat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
      841 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
      901 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
      961 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     1021 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     1081 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     1141 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     1201 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     1261 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     1321 ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     1381 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     1441 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     1501 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     1561 ttcttgacga gttcttctga ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     1621 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     1681 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     1741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1861 atcatgtctg tatttaatta actagaagat gggcgggagt cttctgggca ggcttaaagg
     1921 ctaacctggt gtgtgggcgt tgtcctgcag gggaattgaa caggtgtaaa attggaggga
     1981 caagacttcc cacagatttt cggttttgtc gggaagtttt ttaatagggg caaataggaa
     2041 aatggaggat aggagtcatc tggggtttat gcagcaaaac tacaggtata ttgcttgtat
     2101 ccgcctcgga gatttccatg aggagataaa gacatgtcac ccgagtttat actctcctgc
     2161 ttagatccta ctacagtatg aaatacagtg tngcgaggta gactatgtaa gcagatttaa
     2221 tcattttaaa gagcccagta cttcatatcc atttctcccg ctccttctgc agccttatca
     2281 aaaggtattt agaacactca ttttagcccc attttcattt attatactgg cttatccaac
     2341 ccctagacag agcattggca ttttcccttt cctgatctta gaagtctgat gactcatgaa
     2401 accagacaga ttagttacat acaccacaaa tcgaggctgt agctggggcc tcaacactgc
     2461 agttctttta taactcctta gtacactttt tgttgatcct ttgccttgat ccttaatttt
     2521 cagtgtctat cacctctccc gtcaggtggt gttccacatt tgggcctatt ctcagtccag
     2581 ggagttttac aacaatagat gtattgagaa tccaacctaa agcttaactt tccactccca
     2641 tgaatgcctc tctccttttt ctccattata actgagctat naccattaat ggtttcaggt
     2701 ggatgtctcc tcccccaata tacctgatgt atctacatat tgccaggctg atattttaag
     2761 acatnaaagg tatatttcat tattgagcca catggtattg attactgcta ctaaaatttt
     2821 gtcattgtac acatctgtaa aaggtggttc cttttggaat gcaaagttca ggtgtttgtt
     2881 gtctttcctg acctaaggtc ttgtgagctt gtattttttc tatttaagca gtgctttctc
     2941 ttggactggc ttgactcatg gcattctaca cgttattgct ggtctaaatg tgattttgcc
     3001 aagcttcttc aggacctata attttgcttg acttgtagcc aaacacaagt aaaatgatta
     3061 agcaacaaat gtatttgtga agcttggttt ttaggttgtt gtgttgtgtg tgcttgtgct
     3121 ctataataat actatccagg ggctggagag gtggctcgga gttcaagagc acagactgct
     3181 cttccagaag tcctgagttc aattcccagc aaccacatgg tggctcacaa ccatctgtaa
     3241 tgggatctga tgccctcttc tggtgtgtct gaagaccaca agtgtattca cattaaataa
     3301 ataatcctcc ttcttcttct tttttttttt ttaaagagaa tnctgtctcc agtagaatta
     3361 ctgaagtaat gaaatacttt gtgtttgttc caatatggna gccaataatc aaatactctt
     3421 nagcactgga aatgtaccaa ggaactattt tatttaagtg nactgtggac agaggagcca
     3481 taactgcaga cttgtgggat acagaagacc aatgcagact taatgtcttt tctcttacac
     3541 taagcaataa agaaataaaa attgaacttc tagtatccta tttgttaaac tgctagcttt
     3601 actaactttt gtgcttcatc tatacaaagc tgaaagctaa gtctgcagcc attactaaac
     3661 atgaaagcaa gtaatgataa ttttggattt caaaaatgta gggccagagt ttagccagcc
     3721 agtggtggtg cttgccttta tgccttaatc ccagcactct ggaggcagag acaggcagat
     3781 ctctgagttt gagcccagcc tggtctacac atcaagttct atctaggata gccaggaata
     3841 cacacagaaa ccctgttggg gaggggggct ctgagatttc ataaaattat aattgaagca
     3901 ttccctaatg agccactatg gatgtggcta aatccgtcta cctttctgat gagatttggg
     3961 tattattttt tctgtctctg ctgttggttg ggtcttttga cactgtgggc tttcttaaag
     4021 cctccttccc tgccatgtgg tctcttgttt gctactaact tcccatggct taaatggcat
     4081 ggctttttgc cttctaaggg cagctgctga gntttgcagc ctgatttcca gggtggggtt
     4141 gggaaatctt tcaaacacta aaattgtcct ttaatttttt tttaaaaaat gggttatata
     4201 ataaacctca taaaatagtt atgaggagtg aggtggacta atattaatga gtccctcccc
     4261 tataaaagag ctattaaggc tttttgtctt atactaactt tttttttaaa tgtggtatct
     4321 ttagaaccaa gggtcttaga gttttagtat acagaaactg ttgcatcgct taatcagatt
     4381 ttctagtttc aaatccagag aatccaaatt cttcacagcc aaagtcaaat taagaatttc
     4441 tgactttaat gttatttgct actgtgaata taaaatgata gcttttcctg aggcagggtc
     4501 tcactatgta tctctgcctg atctgcaaca agatatgtag actaaagttc tgcctgcttt
     4561 tgtctcctga atactaaggt taaaatgtag taatactttt ggaacttgca ggtcagattc
     4621 ttttataggg gacacactaa gggagcttgg gtgatagttg gtaaatgtgt ttaagtgatg
     4681 aaaacttgaa ttattatcac cgcaacctac tttttaaaaa aaaaagccag gcctgttaga
     4741 gcatgctaag ggatccctag gacttgctga gcacacaaga gtagtacttg gcaggctcct
     4801 ggtgagagca tatttcaaaa aacaaggcag acaaccaaga aactacagta aggttacctg
     4861 tctttaacca tctgcatata cacagggata ttaaaatatt ccaaataata tttcattcaa
     4921 gttttccccc atcaaattgg gacatggatt tctccggtga ataggcagag ttggaaacta
     4981 aacaaatgtt ggttttgtga tttgtgaaat tgttttcaag tgatagttaa agcccatgag
     5041 atacagaaca aagctgctat ttcgaggtct cttggttata ctcagaagca cttctttggg
     5101 tttccctgca ctatcctgat catgtgctag gcctncctta ggctgattgt tgttcaaata
     5161 acttaagttt cctgtcaggt gatgtcatat gatttcatat atcaaggcaa aacatgttat
     5221 atatgttaaa catttgnact taatgtgaaa gttaggtctt tgtgggtttt gattttaatt
     5281 tcaaaacctg agctaaataa gtcattttac atgtcttaca tttggtgaat tgtatattgt
     5341 ggtttgcagg caagactctc tgacctagta accctcctat agagcacttt gctgggtcac
     5401 aagtctagga gtcaagcatt tcaccttgaa gttgagacgt tttgttagtg tatactagtt
     5461 atatgttgga ggacatgttt atccagaaga tattcaggac tatttttgac tgggctaagg
     5521 aattgattct gattagcact gttagtgagc attgagtggc ctttaggctt gaattggagt
     5581 cacttgtata tctcaaataa tgctggcctt ttttnaaaag cccttgttct ttatcaccct
     5641 gttttctaca taatttttgt tcaaagaaat acttgtttgg atctcctttt gacaacaata
     5701 gcatgttttc aagccatatt ttttttcctt tttttttttt tttttggttt ttcgagacag
     5761 ggtttctctg tatagccctg gctgtcctgg aactcacttt gtagaccagg ctggcctcga
     5821 actcagaaat ccgcctgcct ctgcctcctg agtgccggga ttaaaggcgt gcaccaccac
     5881 gcctggctaa gttggatatt ttgttatata actataacca atactaactc cactgggtgg
     5941 atttttaatt cagtcagtag tcttaagtgg tctttattgg cccttcatta aaatctactg
     6001 ttcactctaa cagaggctgt tggtactagt ggcacttaag caacttccta cggatatact
     6061 agcagattaa gggtcaggga tagaaactag tctagcgttt tgtataccta ccagctttat
     6121 actaccttgt tctgatagaa atatttcagg acatctagct tatcgatacc gtcgacggta
     6181 tcgataagct tgatatcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     6241 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     6301 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     6361 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     6421 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     6481 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     6541 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     6601 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     6661 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcaggtcct
     6721 cgccatggat cctgatgatg ttgttgattc ttctaaatct tttgtgatgg aaaacttttc
     6781 ttcgtaccac gggactaaac ctggttatgt agattccatt caaaaaggta tacaaaagcc
     6841 aaaatctggt acacaaggaa attatgacga tgattggaaa gggttttata gtaccgacaa
     6901 taaatacgac gctgcgggat actctgtaga taatgaaaac ccgctctctg gaaaagctgg
     6961 aggcgtggtc aaagtgacgt atccaggact gacgaaggtt ctcgcactaa aagtggataa
     7021 tgccgaaact attaagaaag agttaggttt aagtctcact gaaccgttga tggagcaagt
     7081 cggaacggaa gagtttatca aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct
     7141 tcccttcgct gaggggagtt ctagcgttga atatattaat aactgggaac aggcgaaagc
     7201 gttaagcgta gaacttgaga ttaattttga aacccgtgga aaacgtggcc aagatgcgat
     7261 gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg
     7321 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     7381 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     7441 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcagatccta
     7501 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     7561 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7621 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7681 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     7741 ctatggcttc tgaggcggaa agaaccagct ggggctcgac ctcgaggggg ggcccggtac
     7801 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     7861 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7921 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7981 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8041 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8101 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8701 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8881 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8941 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9001 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9061 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9121 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9181 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9241 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9301 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9361 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9421 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9481 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9541 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9601 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9661 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9721 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9781 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9841 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9901 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9961 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
    10021 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
    10081 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
    10141 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
    10201 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
    10261 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
    10321 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
    10381 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
    10441 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10501 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10561 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
    10621 gcgcgcgtaa tacgactcac tatagggcga attggagctc cccgcggcag gccctccgag
    10681 cgtggtggag ccgttctgtg agacagccgg gtacgagtcg tgacgctgga aggggcaagc
    10741 gggtggtggg caggaatgcg gtccgccctg cagcaaccgg agggggaggg agaagggagc
    10801 ggaaaagtct ccaccggacg cggccatggc tcgggggggg gggggcagcg gaggancgct
    10861 tccggccgac gtctcgtcgc tgattggctt nttttcctcc cgccgtgtgt gaaaacacaa
    10921 atggcgtgtt ttggttggcg taaggcgcct gtcagttaac ggcagccgga gtgcgcagcc
    10981 gccggcagcc tcgctctgcc cactgggtgg ggcgggaggt aggtggggtg aggcgagctg
    11041 nacgtgcggg cgcggtcggc ctctggcggg gcgggggagg ggagggaggg tcagcgaaag
    11101 tagctcgcgc gcgagcggcc gcccaccctc cccttcctct gggggagtcg ttttacccgc
    11161 cgccggccgg gcctcgtcgt ctgattggct ctcggggccc agaaaactgg cccttgccat
    11221 tggctcgtgt tcgtgcaagt tgagtccatc cgccggccag cgggggcggc gaggaggcgc
    11281 tcccaggttc cggccctccc ctcggccccg cgccgcagag tctggccgcg cgcccctgcg
    11341 caacgtggca ggaagcgcgc gctgggggcg gggacgggca gtagggctga gcggctgcgg
    11401 ggcgggtgca agcacgtttc cgacttgagt tgcctcaaga ggggcgtgct gagccagacc
    11461 tccatcgcgc actccgggga gtggagggaa ggagcgaggg ctcagttggg ctgttttgga
    11521 ggcaggaagc acttgctctc ccaaagtcgc tctgagttgt tatcagtaag ggagctgcag
    11581 tggagtaggc ggggagaagg ccgcaccctt ctccggaggg gggaggggag tgttgcaata
    11641 cctttctggg agttctctgc tgcctcctgg cttctgagga ccgccctggg cctgggagaa
    11701 tcccttgccc cctcttcccc tcgtgatctg caactccagt ctttctagtg gatgcgttcc
    11761 caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga
    11821 aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg
    11881 accatcctcc aaaatcggat ctggttccgc gttctagatc aacaagtttg tacaaaaaag
    11941 caggctggcg ccggaaccaa ttcagtcgac gtttaaacat aacttcgtat agcatacatt
    12001 atacgaagtt atctcgagcc tcaggaaaca gggtaccagt gaaagtctgc aatgaattcg
    12061 agttggcgcg cctgtcattc taaatctctc tttcagccta aagctttttc cccgtatccc
    12121 cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc
    12181 caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg
    12241 ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa
    12301 ttacatccct gggggctttg ggagggggct gtccccgtga gctcccaggc gcgcctgtca
    12361 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
    12421 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
    12481 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
    12541 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
    12601 ttgggggggg gctgtccccg tgagctccca ggcgcgccaa ctcggtaccc ggggatccac
    12661 atatgttaat taagatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
    12721 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
    12781 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
    12841 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
    12901 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
    12961 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
    13021 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
    13081 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
    13141 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
    13201 tgtgaccggc gcctacaagg cctgcaggtc gacggatccg gggaattcat atgtctagat
    13261 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
    13321 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
    13381 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
    13441 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
    13501 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
    13561 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
    13621 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
    13681 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
    13741 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
    13801 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
    13861 aaagtgggtc cgcgtacagc cgcgcgcgta cgaaaaacaa ttacgggtct accatcgagg
    13921 gcctgctcga tctcccggac gacgacgccc ccgaagaggc ggggctggcg gctccgcgcc
    13981 tgtcctttct ccccgcggga cacacgcgca gactgtcgac ggcccccccg accgatgtca
    14041 gcctggggga cgagctccac ttagacggcg aggacgtggc gatggcgcat gccgacgcgc
    14101 tagacgattt cgatctggac atgttggggg acggggattc cccgggtccg ggatttaccc
    14161 cccacgactc cgccccctac ggcgctctgg atatggccga cttcgagttt gagcagatgt
    14221 ttaccgatgc ccttggaatt gacgagtacg gtgggtaggg ggcgcgagga tcctctagag
    14281 ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa
    14341 aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc
    14401 ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac
    14461 aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct
    14521 tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc
    14581 tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt
    14641 agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct
    14701 ggcgtggaaa tattcttatt ggtagaaaca actacaccct ggtcatcatc ctgcctttct
    14761 ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac
    14821 cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
    14881 tgctggttgt tgtgctgtct catcattttg gcaaagaatt cactcctcag gtgcaggctg
    14941 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
    15001 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
    15061 ggtaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
    15121 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
    15181 gtttggcaac atatgccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
    15241 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
    15301 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
    15361 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
    15421 tgtccctctt ctcttatgga gatccctcga ctgcattaat gaatcggcca acgcgcgggg
    15481 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    15541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    15601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    15661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    15721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    15781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    15841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aattagctca cgctgtaggt
    15901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgcgcggccg cagatctcaa
    15961 ctttattata catagttgct taagatctct gaatgcggcc gcttcacaca aaaaaccaac
    16021 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc aggttgggcg
    16081 tcgcttggtc ggtcatttcg aaccccagag tcccgcctgc aggtccggaa ctagtctaag
    16141 tgtcactggg ctgagcagcc accaaaggag aagttgctgg cgggtgacac tgactctccc
    16201 actggccacc acactccact gcatccctta gtacatgtgt ggatccatta atggttgtgg
    16261 ttgaacaagg gacagtctgg cccagaatgg aaggtcttcg gtagcgctca tcatcacagc
    16321 agaggatgat gaggaaggca cgtctaaaag acttattcaa gaaggcgtag agaaaagggt
    16381 tcaacccgga attgatatag ccgagccaga ggaaagcagt ccacacctgc ccagggacag
    16441 tgtagtctat gaaaggatcc acaatattgg tgacaaagaa tggtgcccag cagaggcaga
    16501 agcaacccat gatgatgcat agggtcttgg ctgctttggt ctctgtcctc atgcgatgag
    16561 tgctatgctg gtctgccgac tgaggcctgc tctcggagga ggctcctgcc cgttgtaaca
    16621 tctggatctg atgggcatgc tccttagctg tgacatagat gcggtaatag gccagcacca
    16681 tgaggagaaa tgggatgtag aaggccacca cagagcaggt gatggcgtag ggcttgttga
    16741 ccatgaagac acagtacgta gagttagagt tctggttgaa cttcctcttt tctatcaaat
    16801 caattatgcc aatgttattc cagccttgca ttatagggag aaaagaaata aacgtgggga
    16861 tgacccagca gcctcccagc attaatgcga tgcgcagagg ggtcatcttg ttcctataga
    16921 ccaaaggctg gcagcagatg gcgtaatacc tatccagaga aatgcagcac aggtgaaaaa
    16981 tcgatgccgt tgtgagcagg acggccagag atgtccgaac aagacaaaac acctccccat
    17041 aaatccagat gtcttgaacc agctcaatgg caccaaaggg catcaccagc accgaaacca
    17101 gcagatccgc aaaagcaaga gatacaatga aataatttgt ttttattttc ctgagctgcc
    17161 tgtcccagca cacagccacc atcaccagca ggttccccaa gatggccatc aggataaccg
    17221 tcgagagaaa cgtgagcagc accaccttct ccactgaccc gaaaccctcc tcagaactca
    17281 cattagcatc aagtttgtcc atcctgcagc ccgggggatc cgcccgggcg cggccgctgg
    17341 cgtcatcatc gtccttgtag tcggcgaata ccaggcagaa gatgtagctc agggcgatga
    17401 tcgtcttgat accggtcggt cctgggtttt cttccacatc gcccgcctgt ttcagcaggc
    17461 taaagttggt cgcgccgctg ccctcgagaa accaggtctt gtacagctcg tccatgccgc
    17521 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
    17581 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
    17641 tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc
    17701 tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct
    17761 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
    17821 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
    17881 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
    17941 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact
    18001 gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc ttcagcttgg
    18061 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
    18121 cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt
    18181 tatcctcctc gcccttgctc accatggtgg cggcgcctag gcagtcgacg aattctttgc
    18241 caaaatgatg agacagcaca acaaccagca cgttgcccag gagctgtagg aaagagaaga
    18301 aggcatgaac atggttagca gaggggcccg gtttggactc agagtatttt atcctcatct
    18361 caaacagtgt atatcattgt aaccataaag agaaaggcag gatgatgacc agggtgtagt
    18421 tgtttctacc aataagaata tttccacgcc agccagaatt tatatgcaga aatattctac
    18481 cttatcattt aattataaca attgttctct aaaactgtgc tgaagtacaa tataatatac
    18541 cctgattgcc ttgaaaaaaa agtgattaga gaaagtactt acaatctgac aaataaacaa
    18601 aagtgaattt aaaaattcgt tacaaatgca agctaaagtt taacgaaaaa gttacagaaa
    18661 atgaaaagaa aataagagga gacaatggtt gtcaacagag tagaaagtga aagaaacaaa
    18721 attatcatga gggtccatgg tgatacaagg gacatcttcc cattctaaac aacaccctga
    18781 aaactttgcc ccctccatat aacatgaatt ttacaatagc gaaaaagaaa gaacaatcaa
    18841 gggtccccaa actcaccctg aagttctcag gatccccggg taccgagctc gaattcgggg
    18901 ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt
    18961 ctccaggcga tctgacggtt cactaaacga gctctgctta tataggcctc ccaccgtaca
    19021 cgcctacctc gacccgggta ccgagctcga ctttcacttt tctctatcac tgatagggag
    19081 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
    19141 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
    19201 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
    19261 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
    19321 actgataggg agtggtaaac tcgagtgaag acgaaagggc cttaattaac ctcaggaaac
    19381 agggtaccag tgaaagtctg caatgaattc gagttggcgc gcctgtcatt ctaaatctct
    19441 ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
    19501 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
    19561 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
    19621 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggagggggc
    19681 tgtccccgtg agctcccagg cgcgcctgtc attctaaatc tctctttcag cctaaagctt
    19741 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
    19801 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
    19861 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
    19921 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtcccc gtgagctccc
    19981 aggcgcgcca actcggtacc cggggatcca catatgataa cttcgtatag catacattat
    20041 acgaagttat gtttaaactc gagatatcta gacccagctt tc
//
