LOCUS       sequence-114162-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTrc plasmid expressing p14*-TEV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pJB028
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             390..407
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tgcaggcagc aaaacgtgca aatattcgtc tgcctccgga agttaatcgc attctgtata
       61 ttcgtaatct gccgtacaaa atcacagccg aagaaatgta tgatatcttt ggcaaatatg
      121 gtccgattcg tcagattcgt gttggtaata caccggaaac ccgtggcacc gcatatgttg
      181 tttatgaaga tattttcgac gccaaaaacg cctgtgatca tctgagcggt tttaatgttt
      241 gtaatcgtta tctggtggtg ctgtattata acgcaaatcg tgcattccag aaaatggata
      301 ccaaaaaaaa agaagaacag ctgaaactgc tgaaagaaaa atatggcatc aatacctacc
      361 ctccgaaaat ggttaatccg atgagcggtc tggttccgcg tggtagcgca atgggtgaaa
      421 gcctgtttaa aggtccgcgt gattataacc cgattagcag cgatatttgt catctgacca
      481 atgaaagtga tggtcatacc accagtctgt atggtattgg ttttggtccg tttatcatca
      541 ccaacaaaca cctgtttcgt cgtaataatg gcaccctgct ggttcagagc ctgcatggtg
      601 tttttaaagt taaagatacc accaccctgc agcagcatct ggttgatggt cgtgatatga
      661 tgattattcg tatgccgaaa gattttccgc cttttccgca g
//
