LOCUS       sequence-114163-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and 
            nontargeting CRISPR array with BsaI site for inserting user-defined 
            spacer-repeat bricks.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Cress BF, Toparlak OD, Guleria S, Lebovich M, Stieglitz JT, 
            Englaender JA, Jones JA, Linhardt RJ, Koffas MA
  TITLE     CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR 
            Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E.
            coli.
  JOURNAL   ACS Synth Biol. 2015 Mar 30.
  PUBMED    25822415
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Mar 30."
COMMENT     Sequence Label: pCRISPathBrick
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(323..401)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc
       61 taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt
      121 ggttatgccg gtactgccgg gcctcttgcg ggattacgaa atcatcctgt ggagcttagt
      181 aggtttagca agatggcagc gcctaaatgt agaatgataa aaggattaag agattaattt
      241 ccctaaaaat gataaaacaa gcgttttgaa agcgcttgtt tttttggttt gcagtcagag
      301 tagaatagaa gtatcaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
      361 ctagccttat tttaacttgc tatgctgttt tgaatggttc caacaagatt attttataac
      421 ttttataaca aataatcaag gagaaattca aagaaattta tcagccataa aacaatactt
      481 aatactatag aatgataaca aaataaacta ctttttaaaa gaattttgtg ttataatcta
      541 tttattatta agtattgggt aatatttttt gaagagatat tttgaaaaag aaaaattaaa
      601 gcatattaaa ctaatttcgg aggtcattaa aactattatt gaaatcatca aactcattat
      661 ggatttaatt taaacttttt attttaggag gcaaaaatgg ataagaaata ctcaataggc
      721 ttagctatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata taaggttccg
      781 tctaaaaa
//
