LOCUS       sequence-114170-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTrc plasmid expressing clpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pKS043
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      496..582
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgaacgactg gctggacttt gatcaactgg cggaagaaaa agttcgcgac gcgctaaaac
       61 cgccatctat gtataaagtg atattagtca atgatgatta cactccgatg gagtttgtta
      121 ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg tgcaacgcaa ttgatgctcg
      181 ctgttcacta ccaggggaag gccatttgcg gagtctttac cgccgaggtt gcagaaacca
      241 aagtggcgat ggtgaacaag tacgcgaggg agaatgagca tccattgctg tgtacgctag
      301 aaaaagcctg agagctcgag atctgcagct ggtaccatat gggaattcga agctttctag
      361 aactagtaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
      421 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
      481 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      541 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
      601 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
      661 catcaaatta agcagaaggc catcctgacg gatggcc
//
