LOCUS       sequence-114172-        2765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - Controller protein, tetR repressor 
            (represses pTet, C0040. can be inhibited by tetracyclin or aTc) 
            [C:C0040:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: C0040 (tetR)_CD
FEATURES             Location/Qualifiers
     source          1..2765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..622
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(75..94)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             623..655
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     misc_feature    673..693
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(699..716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(870..1458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(950..969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1629..2420,2421..2489))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2252..2271
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2490..2594)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2662..2680
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2684..2727)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2730..2751
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
       61 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      121 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      181 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      241 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      301 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      361 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      421 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      481 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      541 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      601 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      661 aaggtagaga cctactagta gcggccgctg caggcttcct cgctcactga ctcgctgcgc
      721 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      781 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      841 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
      901 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      961 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1021 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1081 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1141 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1201 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1261 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1321 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1381 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1441 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1501 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1561 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1621 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1681 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1741 tctggcccca gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca
     1801 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1861 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     1921 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     1981 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2041 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2101 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2161 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2221 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2281 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2341 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2401 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2461 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2521 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2581 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2641 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     2701 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga gactagtggg
     2761 tctca
//
