LOCUS       sequence-114177-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTrc plasmid expressing GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pJB005
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(20..48)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     643..663
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
       61 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      121 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      181 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      241 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      301 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      361 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      421 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      481 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      541 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      601 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      661 aacagctgct gggattacac atggcatgga tgaactatac aaaaggcctg cagcaaacga
      721 cgaaaactac gctgcatcag tttaagagct cgagatctgc agctggtacc atat
//
