LOCUS       sequence-114178-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTrc plasmid expressing GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pJB005
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(26..117)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(136..163)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(255..341)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(608..628)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
       61 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaaaga
      121 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg
      181 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa
      241 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
      301 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac
      361 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg
      421 catggggtca ggtgggacca ccgcgctact gccgccaggc aaattctgta ctagttctag
      481 aaagcttcga attcccatat ggtaccagct gcagatctcg agctcttaaa ctgatgcagc
      541 gtagttttcg tcgtttgctg caggcctttt gtatagttca tccatgccat gtgtaatccc
      601 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
      661 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
      721 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct
//
