LOCUS       sequence-114185-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This a retroviral expression plasmid with a minimal polylinker 
            followed by IRES and hygro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 7749 Bicistronic_ires_hygro
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
       61 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaaaaaca cgatgataat atggccacaa gattcgaggt tggggggcaa tgagatatga
      181 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
      241 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
      301 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
      361 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
      421 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
      481 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
      541 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
      601 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
      661 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
      721 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
      781 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
      841 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
      901 tggagcagca gacgcgctac ttcgagcgga
//
