LOCUS       sequence-114186-         280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This a retroviral expression plasmid with a minimal polylinker 
            followed by IRES and hygro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 7749 Bicistronic_ires_hygro
FEATURES             Location/Qualifiers
     source          1..280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     170..192
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     208..224
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
       61 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
      121 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
      181 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
      241 ggaattagat ctctcgaggt taaccgcccc ccccccctaa
//
