LOCUS       sequence-114187-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This a retroviral expression plasmid expressing GFP tagged G12C 
            mutant Kras 2A oncogene along with puro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 8024 GFP_Kras_G12C_2A_puro
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtccggact cagatctatg actgagtata aacttgtggt ggttggagct tgtggcgtag
       61 gcaagagcgc cttgacgata cagctaattc agaatcactt tgtggatgag tacgacccta
      121 cgatagagga ctcctacagg aaacaagtag taattgatgg agaaacctgt ctcttggata
      181 ttctcgacac agcaggtcaa gaggagtaca gtgcaatgag ggaccagtac atgagaactg
      241 gggagggctt tctttgtgta tttgccataa ataatactaa atcatttgaa gatattcacc
      301 attatagaga acaaattaaa agagtaaagg actctgaaga tgtgcctatg gtcctggtag
      361 ggaataagtg tgatttgcct tctagaacag tagacacgaa acaggctcag gagttagcaa
      421 ggagttacgg gattccgttc attgagacct cagcaaagac aagacagaga gtggaggatg
      481 ctttttatac attggtgaga gagatccgac agtacagatt gaaaaaaatc agcaaagaag
      541 aaaagactcc tggctgtgtg aaaattaaaa aatgcgttat aatgtaaaag ggcgaattct
      601 gcagatatcc atcacactgg cggccgctcg acggtaccgc gggcccaacg aattccgccc
      661 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
      721 atgttatttt ccaccatatt gccgtctttt ggcnatgtga gggcccggaa acctggccct
      781 gtcttcttga cgagcattcc taggggtctt tcccctctc
//
