LOCUS       sequence-114189-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This a retroviral expression plasmid expressing GFP along with puro 
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 7432 Bicistronic_GFP_ires_puro
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(50..52,53..55,56..769)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(50..52,53..55,56..766)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(95..116)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(356..375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     703..724
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatccaccg gtcgccacca tggtgagcaa
       61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      181 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      241 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      481 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      541 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      661 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgctc
      781 gaggttaacg aattccgccc ccccccccta acgttactgg ccgaa
//
