LOCUS       sequence-114192-         285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This a retroviral expression plasmid with a minimal polylinker 
            followed by IRES and puro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 6691 Bicistronic_ires_puro
FEATURES             Location/Qualifiers
     source          1..285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     169..191
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     207..223
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
       61 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
      121 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
      181 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
      241 gaattagatc tctcgaggtt aacgaattcc gccccccccc cctaa
//
