LOCUS       sequence-114194-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This a retroviral expression plasmid expressing GFP tagged G12C 
            mutant Kras 2B oncogene along with puro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 8027_GFP-KrasG12C_2B_puro
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..652)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(136..156)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     633..652
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac
       61 cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga
      121 cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg
      181 cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac
      241 ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg
      301 gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca
      361 actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg
      421 tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc
      481 cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat
      541 cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc
      601 tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggttgtgg
      661 ccatattatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga
      721 cgttttttta acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat
      781 cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttga
//
