LOCUS       sequence-114200-        3023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - Controller protein, araC repressor/activator
            (activates pBAD, I13453) [C:C0080:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: C0080 (araC)_CD
FEATURES             Location/Qualifiers
     source          1..3023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..880
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             881..913
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     misc_feature    931..951
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(957..974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1128..1716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1208..1227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1887..2678,2679..2747))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2510..2529
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2748..2852)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2920..2938
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2942..2985)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2988..3009
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatggct gaagcgcaaa atgatcccct gctgccggga tactcgttta acgcccatct
       61 ggtggcgggt ttaacgccga ttgaggccaa tggttatctc gattttttta tcgaccgacc
      121 gctgggaatg aaaggttata ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa
      181 tcagggacga gaatttgtct gccgaccggg tgatattttg ctgttcccgc caggagagat
      241 tcatcactac ggtcgtcatc cggaggctcg cgaatggtat caccagtggg tttactttcg
      301 tccgcgcgcc tactggcatg aatggcttaa ctggccgtca atatttgcca atacgggttt
      361 ctttcgcccg gatgaagcgc accagccgca tttcagcgac ctgtttgggc aaatcattaa
      421 cgccgggcaa ggggaagggc gctattcgga gctgctggcg ataaatctgc ttgagcaatt
      481 gttactgcgg cgcatggaag cgattaacga gtcgctccat ccaccgatgg ataatcgggt
      541 acgcgaggct tgtcagtaca tcagcgatca cctggcagac agcaattttg atatcgccag
      601 cgtcgcacag catgtttgcc tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt
      661 agggattagc gtcttaagct ggcgcgagga ccaacgcatc agccaggcga agctgctttt
      721 gagcactacc cggatgccta tcgccaccgt cggtcgcaat gttggttttg acgatcaact
      781 ctatttctcg cgagtattta aaaaatgcac cggggccagc ccgagcgagt tccgtgccgg
      841 ttgtgaagaa aaagtgaatg atgtagccgt caagttgtca gctgcaaacg acgaaaacta
      901 cgctttagta gcttaataaa ggtagagacc tactagtagc ggccgctgca ggcttcctcg
      961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
     1141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1561 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgcga cccacgctca
     2041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2881 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     2941 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     3001 cttctagaga ctagtgggtc tca
//
