LOCUS       sequence-114203-        3139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP 
            sites) [C:cre:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: cre_CD
FEATURES             Location/Qualifiers
     source          1..3139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1033
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     KYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    1047..1067
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1073..1090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1244..1832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1324..1343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2003..2794,2795..2863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2626..2645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2864..2968)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3036..3054
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3058..3101)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3104..3125
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgtccaat ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag
       61 tgatgaggtt cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca
      121 tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa
      181 ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc
      241 gcgcggtctg gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg
      301 tcggtccggg ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcgaat
      361 ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac
      421 tgatttcgac caggttcgtt cactcatgga aaatagcgat cgctgccagg atatacgtaa
      481 tctggcattt ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat
      541 cagggttaaa gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac
      601 gaaaacgctg gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact
      661 ggtcgagcga tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg
      721 ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc
      781 cctggaaggg atttttgaag caactcatcg attgatttac ggcgctaagg atgactctgg
      841 tcagaaatac ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc
      901 ccgcgctgga gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat
      961 tgtcatgaac tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga
     1021 agatggcgat taataaggta gagacctact agtagcggcc gctgcaggct tcctcgctca
     1081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
     1261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2101 gggagggctt accatctggc cccagtgctg caatgatacc gcgcgaccca cgctcaccgg
     2161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     3001 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt
     3061 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc
     3121 tagagactag tgggtctca
//
