LOCUS       sequence-114205-        2831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - Fluorescent protein. Yellow.  [C:E0030:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: E0030 (YFP)_CD
FEATURES             Location/Qualifiers
     source          1..2831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(5..7,8..10,11..724)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(50..71)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(311..330)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     658..679
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    739..759
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(765..782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(936..1524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1016..1035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1695..2486,2487..2555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2318..2337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2556..2660)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2728..2746
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2750..2793)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2796..2817
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
       61 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      121 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      181 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat
      241 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      301 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      361 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      421 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      481 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      541 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      601 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      661 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      721 gtaataaagg tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg
      781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
      961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1621 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1681 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1741 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1801 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
     1861 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1921 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1981 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2041 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2101 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2161 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2221 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2281 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2341 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2401 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2461 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2521 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2581 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2641 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2701 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     2761 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact
     2821 agtgggtctc a
//
