LOCUS       sequence-114207-        2828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - Fluorescent protein. Green. Modified from 
            Bba_E0040 to fix illegal site. [C:E0040m:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: E0040m (GFP)_CD
FEATURES             Location/Qualifiers
     source          1..2828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..718
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(35..63)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     658..678
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    736..756
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(762..779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(933..1521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1013..1032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1692..2483,2484..2552))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2315..2334
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2553..2657)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2725..2743
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2747..2790)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2793..2814
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
       61 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      121 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      181 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
      241 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      301 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      361 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      421 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      481 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      541 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      601 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag atcacatggt
      661 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
      721 ataaaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg
      781 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      841 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      901 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
      961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1681 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1741 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1801 ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta
     1861 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1921 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1981 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2041 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2101 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2161 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2221 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2281 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2341 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2401 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2461 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2521 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2581 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2641 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2701 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     2761 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagt
     2821 gggtctca
//
