LOCUS       sequence-114215-        2864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - Controller protein, luxR repressor/activator
            (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates 
            pLuxR(pL) R0062) [C:C0062:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: C0062 (luxR)_CD
FEATURES             Location/Qualifiers
     source          1..2864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    772..792
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(798..815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(969..1557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1049..1068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1728..2519,2520..2588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2351..2370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2589..2693)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2761..2779
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2783..2826)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2829..2850
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg
       61 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga
      121 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct
      181 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga
      241 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa
      301 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat
      361 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc
      421 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc
      481 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc
      541 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag
      601 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac
      661 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt
      721 aacaggagca attgattgcc catactttaa aaattaataa aggtagagac ctactagtag
      781 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      841 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      901 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      961 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1021 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1081 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1141 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1201 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1261 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1321 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1381 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1441 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1501 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1561 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1621 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1681 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1741 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1801 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1861 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1921 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1981 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2041 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2101 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2161 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2221 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2281 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2341 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2401 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2461 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2521 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2581 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2641 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2701 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2761 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2821 gatttctgga attcgcggcc gcttctagag actagtgggt ctca
//
