LOCUS       sequence-114218-        2237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: Double terminator (B0010:B0012)  [D:B0015:E].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: B0015_DE
FEATURES             Location/Qualifiers
     source          1..2237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      13..84
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      100..127
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    145..165
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(171..188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(342..930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(422..441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1101..1892,1893..1961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1724..1743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1962..2066)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2134..2152
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2156..2199)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2202..2223
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aggtccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
       61 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      121 ttctgcgttt atagcttaga gacctactag tagcggccgc tgcaggcttc ctcgctcact
      181 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      241 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
      361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1081 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1201 gagggcttac catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct
     1261 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1381 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1621 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1741 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1801 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1861 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1921 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1981 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2041 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2101 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
     2161 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta
     2221 gagactagtg ggtctca
//
