LOCUS       sequence-11422-e        8865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Lu J, Beatty L, Pinthus J
  TITLE     Dual expression recombinase based (DERB) single vector system for 
            high throughput screening and verification of protein interactions 
            in living cells
  JOURNAL   Nat Prec (2008).
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Prec 
            (2008)."
COMMENT     Sequence Label: Y pFe-YcLoxP-YnAtt
FEATURES             Location/Qualifiers
     source          1..8865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          700..1644
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1601..1621
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1710..1728
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1731..1749
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1759..1783
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1886..1888,1889..1891,1892..2350)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(1931..1952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2192..2211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    2363..2487
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2512..2517,2518..2535,2536..2542)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2596..3255
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2662..2681)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3597..3902
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3781..3800
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3943..4067)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4129..4147)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4158..4175)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4158..4174)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4167..4189)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    4215..4336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4226..4245)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4280..4299
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4524..5112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5013..5032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5234..5425
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5257..5277)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          5632..6579
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     primer_bind     6825..6846
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6859..6892)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6907..7351
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7385..7431
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7423..7441
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7451..8245
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7451..8245
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7505..7524)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8115..8134
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8292..8864
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      121 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      181 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      241 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      301 gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      361 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttgactagtc
      421 agggccccaa cccccccaag cccccatttc acaacacgct ggcgctacag gcgcgtgact
      481 tccccttgct ttggggcggg gggctgagac tcctatgtgc tccggattgg tcaggcacgg
      541 ccttcggccc cgcctcctgc caccgcagat tggccgctag gcctccccga gcgccctgcc
      601 tccgagggcc ggcgcaccat aaaagaagcc gccctagcca cgtcccctcg cagttcggcg
      661 gtcccgcggg tctgtctcaa gcttgccgcc agaacacagg taagtgccgt gtgtggttcc
      721 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccatgccc
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg
      901 ctggggccgc cgcgtgctaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgctaa
      961 gtctctagcc atttaaaatt tttgataacc agctgcgacg ctttttttct ggcgagatag
     1021 tcttgtaaat gcgggccagg atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaaac tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 cccgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cttcctcatc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttgtc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagagtt aggccagctt ggcacttgat gtaattctcc
     1561 ttggaatttg ccctttttga gtttggatct tgcctcattc tcaagcctca gacagtggtt
     1621 caaagttttt ttcttccatt tcaggtgtcg tgaaaactac ccctaaaagc caccggcgtg
     1681 cgcaagatca gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
     1741 tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     1801 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagagga
     1861 tccctaccgg tgaaggagat ataccatggt gagcaagggc gaggagctgt tcaccggggt
     1921 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1981 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2041 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     2101 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2161 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2221 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2281 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2341 tatcatggcc tcgagcccat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     2401 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     2461 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
     2521 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga
     2581 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     2641 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     2701 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     2761 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     2821 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     2881 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     2941 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     3001 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     3061 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     3121 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     3181 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     3241 cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca gtatgcgtat
     3301 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     3361 aaaagaggtg tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     3421 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     3481 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     3541 cgcccggttt attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc
     3601 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     3661 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     3721 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     3781 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     3841 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     3901 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     3961 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     4021 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga tgggcggccg
     4081 ctctagaggg cccaagctta cgcgtgcatg cgacgtcata gctctctccc tatagtgagt
     4141 cgtattataa gctaggcact ggccgtcgtt ttacaacgtc gtgactggga aaactgctag
     4201 ctggccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4261 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4321 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4381 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg aaatgttaat
     4441 taactagcca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4501 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4561 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4621 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4681 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4741 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4801 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4861 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4921 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4981 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5041 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5101 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5161 tttgctcaca tgttcttaat taacctgcag ggcctgaaat aacctctgaa agaggaactt
     5221 ggttaggtac cttctgaggc tgaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5281 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5341 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5401 tcaattagtc agcaaccata gtcccactag ttccgccaga gcgcgcgagg gcctccagcg
     5461 gccgcccctc ccccacagca ggggcggggt cccgcgccca ccggaaggag cgggctcggg
     5521 gcgggcggcg ctgattggcc ggggcgggcc tgacgccgac gcggctataa gagaccacaa
     5581 gcgacccgca gggccagacg ttcttcgccg aagcttgccg tcagaacgca ggtgaggggc
     5641 gggtgtggct tccgcgggcc gccgagctgg aggtcctgct ccgagcgggc cgggccccgc
     5701 tgtcgtcggc ggggattagc tgcgagcatt cccgcttcga gttgcgggcg gcgcgggagg
     5761 cagagtgcga ggcctagcgg caaccccgta gcctcgcctc gtgtccggct tgaggcctag
     5821 cgtggtgtcc gcgccgccgc cgcgtgctac tccggccgca ctctggtctt tttttttttt
     5881 gttgttgttg ccctgctgcc ttcgattgcc gttcagcaat aggggctaac aaagggaggg
     5941 tgcggggctt gctcgcccgg agcccggaga ggtcatggtt ggggaggaat ggagggacag
     6001 gagtggcggc tggggcccgc ccgccttcgg agcacatgtc cgacgccacc tggatggggc
     6061 gaggcctggg gtttttcccg aagcaaccag gctggggtta gcgtgccgag gccatgtggc
     6121 cccagcaccc ggcacgatct ggcttggcgg cgccgcgttg ccctgcctcc ctaactaggg
     6181 tgaggccatc ccgtccggca ccagttgcgt gcgtggaaag atggccgctc ccgggccctg
     6241 ttgcaaggag ctcaaaatgg aggacgcggc agcccggtgg agcgggcggg tgagtcaccc
     6301 acacaaagga agagggcctg gtccctcacc ggctgctgct tcctgtgacc ccgtggtcct
     6361 atcggccgca atagtcacct cgggcttttg agcacggcta gtcgcggcgg ggggagggga
     6421 tgtaatggcg ttggagtttg ttcacatttg gtgggtggag actagtcagg ccagcctggc
     6481 gctggaagtc atttttggaa tttgtcccct tgagttttga gcggagctaa ttctcgggct
     6541 tcttagcggt tcaaaggtat cttttaaacc cttttttagg tgttgtgaaa accaccgcta
     6601 attcaaagca accggtgaag gagatatatc atggccgaca agcagaagaa cggcatcaag
     6661 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     6721 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     6781 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     6841 ttcgtgaccg ccgccgggat aacttcgtat agcatacatt atacgaagtt ataggtcgac
     6901 cctaggagca ggtttcccca atgacacaaa acgtgcaact tgaaactccg cctggtcttt
     6961 ccaggtctag aggggtaaca ctttgtactg cgtttggctc cacgctcgat ccactggcga
     7021 gtgttagtaa cagcactgtt gcttcgtagc ggagcatgac ggccgtggga actcctcctt
     7081 ggtaacaagg acccacgggg ccaaaagcca cgcccacacg ggcccgtcat gtgtgcaacc
     7141 ccagcacggc gactttactg cgaaacccac tttaaagtga cattgaaact ggtacccaca
     7201 cactggtgac aggctaagga tgcccttcag gtaccccgag gtaacacgcg acactcggga
     7261 tctgagaagg ggactggggc ttctataaaa gcgctcggtt taaaaagctt ctatgcctga
     7321 ataggtgacc ggaggtcggc acctttcctt tgcaattact gaccctatga atacaactga
     7381 ctgtttgaca attaatcatc ggcatagtat atcggcatag tataatacga ctcactatag
     7441 gagggccacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     7501 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     7561 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     7621 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     7681 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     7741 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7801 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7861 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7921 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     7981 catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat
     8041 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     8101 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     8161 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     8221 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     8281 aattcgctag gattatccct aatacctgcc accccactct taatcagtgg tggaagaacg
     8341 gtctcagaac tgtttgtttc aattggccat ttaagtttag tagtaaaaga ctggttaatg
     8401 ataacaatgc atcgtaaaac cttcagaagg aaaggagaat gttttgtgga ccactttggt
     8461 tttctttttt gcgtgtggca gttttaagtt attagttttt aaaatcagta ctttttaatg
     8521 gaaacaactt gaccaaaaat ttgtcacaga attttgagac ccattaaaaa agttaaatga
     8581 gaaacctgtg tgttcctttg gtcaacaccg agacatttag gtgaaagaca tctaattctg
     8641 gttttacgaa tctggaaact tcttgaaaat gtaattcttg agttaacact tctgggtgga
     8701 gaatagggtt gttttccccc cacataattg gaaggggaag gaatatcatt taaagctatg
     8761 ggagggttgc tttgattaca acactggaga gaaatgcagc atgttgctga ttgcctgtca
     8821 ctaaaacagg ccaaaaactg agtccttggg ttgcatagaa agctg
//
